Anti-Human EGF (Clone 10827)

Pricing & Details

Product No.E256
Clone
10827
Protein
EGF
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Epidermal Growth Factor, Urogastrone, URG, C-erbB
Isotype
Mouse
IgG1
Applications
ELISA Cap
,
IHC FFPE
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
E256-25 µg
25 µg
$185.00
In stock
Max:
Min: 1
Step: 1
E256-100 µg
100 µg
$389.00
In stock
Max:
Min: 1
Step: 1
E256-500 µg
500 µg
$920.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human EGF (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:E121 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:E108.
Other Applications Reported In Literature ?
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 25 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Epidermal Growth Factor (EGF) (Clone 10827) recognizes an epitope on Human EGF. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR.1 EGF acts by binding with high affinity to epidermal growth factor receptor (EGFR) on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. This causes variety of biochemical changes within the cell - a rise in intracellular calcium levels, increased glycolysis and protein synthesis, and increases in the expression of certain genes including the gene for EGFR - that ultimately lead to DNA synthesis and cell proliferation.2 EGF has been shown to regulate tumor cell invasion through activation of matrix metalloproteinase-2 (MMP-2) in various tumor cell types.3

Antigen Details

Protein
PubMed
NCBI Gene Bank ID

References & Citations

1. Cohen, S. et al. (1995) J. Biol. Chem 265: 7709
2. Seroogy, KB. et al. (1995) Science 224: 1107
3. Cosen-Binker, LI. et al. (2009) Biochem Biophys Res Commun. 379: 445
Elisa Sandwich Protocol
IHC
General Western Blot Protocol

Formats Available

Prod No.
Description
E256
Products are for research use only. Not for use in diagnostic or therapeutic procedures.