Anti-Rat GFRα-1
Anti-Rat GFRα-1
Product No.: G682
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Target GDNF R alpha-1 Product Type Polyclonal Antibody Alternate Names Glial Cell Line-Derived Neurotropic Factor soluble Receptor Alpha 1, GFRA1, GDNFR, GDNFRA, GFR-ALPHA-1, MGC23045, RET1L, RETL1, TRNR1 Applications B , IHC FFPE , WB |
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Antibody DetailsProduct DetailsReactive Species Rat Host Species Goat Immunogen NS0 - Derived Soluble Recombinant Rat GFRα-1 Extracellular Domain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830271 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Rat GFRα-1 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Rat GFRα-1 is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 1 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Blocking:In a functional ELISA, 4-10 µg/mL of this antibody will block 50% of the binding of 4 ng/mL of Recombinant Human GDNF to immobilized Recombinant Rat GFR alpha -1 Fc Chimera coated at 0.5 µg/mL (100 µL/well). Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat Anti-Rat Glial Cell-Line Derived Neurotrophic Factor Soluble Receptor Alpha 1 (GFRα-1) recognizes Rat GFRα-1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background GDNF family receptor alpha 1, also known as GFRA1 is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRalpha1 functions as a ligand-induced cell adhesion molecule (LICAM) to establish precise synaptic contacts and induce presynaptic differentiation.1 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Ibáñez, CF. et al. (2007)Nat Neurosci. 10:293 Technical ProtocolsB IHC FFPE  Certificate of Analysis |
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