Anti-Rat Kappa Light Chain – Purified in vivo GOLD™ Functional Grade

Anti-Rat Kappa Light Chain – Purified in vivo GOLD™ Functional Grade

Product No.: I-2027

[product_table name="All Top" skus="I-1188"]

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Clone
MAR 18.5
Target
Kappa Light Chain
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
Mouse IgG2a k
Applications
ELISA
,
FA
,
in vivo
,
WB

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Product Size
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Antibody Details

Product Details

Reactive Species
Rat
Host Species
Mouse
Immunogen
Soluble rat immunoglobulin
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% by SDS Page
≥95% monomer by analytical SEC
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
MAR 18.5 activity is directed against rat kappa immunoglobulin light chain of both RI-1a and RI-1b allotypes.
Antigen Distribution
Immunoglobulins consist of heavy chains and light chains. Kappa is a class of light chain and is encoded by the V (variable), J (joining), and C (constant) segments.
Background
MAR 18.5 is a monoclonal antibody directed against rat kappa light chains1. MAR 18.5 was generated by immunizing SJL/J mice with soluble rat immunoglobulin, followed by the creation of a B cell hybridoma line via fusion of immune spleen with P3X63Ag8 myeloma cells. MAR 18.5 hybridoma cells secrete an IgG2a kappa monoclonal antibody that strongly binds to protein A. Additionally, MAR 18.5 antibody binds similarly to Ig of RI-1a and RI-1b allotypes. MAR 18.5 antibody can be used in combination with anti-CD19 and anti-CD22 for in vivo B cell depletion in mice2,3. In a study on Fcγ receptor-mediated phagocytosis, MAR 18.5 antibody was used as a secondary cross-linking antibody during stimulation of macrophages grown in medium lacking L cell–conditioned medium (LCM) and treated with chilled supernatant from the rat anti-FcγR 2.4G2 hybridoma4. Additionally, MAR 18.5 antibody has been used for T cell isolation and complement lysis in combination with J11d.2 (anti-heat-stable Ag), 2.43 (anti-CD8), M5/114 (anti-class II), and 2.4G2 (anti-FcR)5.

Antigen Details

References & Citations

1. Lanier LL, Gutman GA, Lewis DE, et al. Hybridoma. 1(2):125-131. 1982.
2. Säwén P, Lang S, Mandal P, et al. Cell Rep.;14(12):2809-2818. 2016.
3. Keren Z, Naor S, Nussbaum S, et al. Blood. 117(11):3104-3112. 2011.
4. Fitzer-Attas CJ, Lowry M, Crowley MT, et al. J Exp Med. 191(4):669-682. 2000.
5. Hurst SD, Sitterding SM, Ji S, Barrett TA. Proc Natl Acad Sci U S A. 94(8):3920-3925. 1997.
6. Nilsson G, Matsson P, Ahlstedt S. Scand J Immunol. 31(1):53-57. 1990.
7. Elbe-Bürger A, Mommaas AM, Prieschl EE, et al. Immunology. 101(2):242-253. 2000.
8. Zheng Y, Zhou ZZ, Lyttle CR, et al. J Leukoc Biol. 44(1):27-32. 1988.
9. Zhou ZZ, Zheng Y, Steenstra R, et al. Autoimmunity. 3(2):125-134. 1989.
10. Jonsson CA, Carlsten H. Int Immunopharmacol. 3(1):31-37. 2003.
11. Mpandi M, Otten LA, Lavanchy C, et al. J Virol. 77(17):9369-9377. 2003.
12. Reitan SK, Hannestad K. Proc Natl Acad Sci U S A. 99(11):7588-7593. 2002.
Indirect Elisa Protocol
FA
in vivo Protocol
General Western Blot Protocol

Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.