Anti-Respiratory Syncytial Virus (Clone: RSV-12I1)

Pricing & Details

Product No.LT559
Clone
RSV-12I1
Protein
RSV
Formats AvailableView All
Product Type
Recombinant Monoclonal Antibody
Alternate Names
RSV, Orthopneumovirus
Isotype
Human IgG1λ
Applications
ELISA
,
N
Prod No.
Size
Price
Avail.
Qty
Add to cart
LT559-250 µg
250 µg
$390.00
Pre-order
Max:
Min: 1
Step: 1
LT559-1.0 mg
1.0 mg
$1,400.00
Pre-order
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Respiratory Syncytial
Virus
Expression Host
HEK-293
Immunogen
Human donors targeting the post-fusion RSV F protein using human hybridoma technology
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
Formulation
This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one year. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≥ -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Standard Overnight on Blue Ice.
Other Applications Reported In Literature ?
ELISA

NOTE: RSV-12I1 failed to show neutralization activity against RSV strain A2 in a plaque reduction neutralization assay.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
RSV-12I1 activity is directed against antigenic site II of the RSV fusion (F) protein.

Clone RSV-12I1 did bind to both pre- and post-fusion F protein in an ELISA binding assay, favoring the post-fusion conformation. Competition-binding studies showed that RSV-12I1 targets antigenic site II, which is the target of palivizumab, an antiviral monoclonal antibody licensed as a prophylactic treatment. RSV-12I1 readily competed with RSV-14N4 on post-fusion F, but the competition was less pronounced on pre-fusion F. RSV-12I1 also competed with palivizumab on post-fusion F in a palivizumab competition assay. Saturation alanine scanning mutagenesis identified residues Leu467 and Lys470 as critical for RSV-12I1 binding. Binding was not detected to scaffolded epitopes containing site II.
Antigen Distribution
F protein is a surface glycoprotein
Background
Respiratory syncytial virus (RSV) is a common respiratory virus that infects the majority of children by two years old1, 2. While usually mild, RSV can be serious in infants and older adults and is the leading cause of bronchiolitis and pneumonia in children less than one year of age in the United States1. Antibodies have been described that bind and neutralize RSV fusion (F) protein2-4.

RSV F protein is a type I integral membrane protein that is synthesized as a 574 amino acid inactive precursor, assembled into a trimer, post-translationally modified, then cleaved to produce F1, F2, and intervening peptide pep273. Functional F protein has both pre- and post-fusion conformations. RSV F protein is highly conserved among RSV isolates from both A and B subgroups3 and is the primary target for antiviral drug development3 with several antigenic regions capable of introducing neutralizing antibodies2.

Antigen Details

Protein
PubMed
Research Area
Infectious Disease
.
Respiratory Syncytial
.
Viral

References & Citations

1. National Center for Immunization and Respiratory Diseases (NCIRD), Division of Viral Diseases, Link Text
2. Mousa JJ, Binshtein E, Human S, et al. PLoS Pathog. 14(2):e1006837. 2018.
3. McLellan JS, Ray WC, Peeples ME. Curr Top Microbiol Immunol. 372:83-104. 2013.
4. Mousa JJ, Sauer MF, Sevy AM, et al. Proc Natl Acad Sci U S A. 113(44):E6849-E6858. 2016.
Elisa Sandwich Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.