Anti-Respiratory Syncytial Virus (Clone: RSV-3M3)

Pricing & Details

Product No.LT556
Clone
RSV-3M3
Protein
RSV
Formats AvailableView All
Product Type
Recombinant Monoclonal Antibody
Alternate Names
RSV, Orthopneumovirus
Isotype
Human IgG1λ
Applications
ELISA
,
N
Prod No.
Size
Price
Avail.
Qty
Add to cart
LT556-250 µg
250 µg
$390.00
Pre-order
Max:
Min: 1
Step: 1
LT556-1.0 mg
1.0 mg
$1,400.00
Pre-order
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Respiratory Syncytial
Virus
Expression Host
HEK-293
Immunogen
Human donors targeting the post-fusion RSV F protein using human hybridoma technology
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
Formulation
This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one year. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≥ -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Standard Overnight on Blue Ice.
Other Applications Reported In Literature ?
ELISA
N
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
RSV-3M3 activity is directed against antigenic site IV of the RSV fusion (F) protein and binds to pre- and post-fusion F proteins of RSV A strain A2 and RSV B strain 18537.

Clone RSV-3M3 is potent at neutralizing RSV A2, RSV Long viruses (subgroup A), RSV 18537 B, and RSV WV/401R viruses (subgroup B).

Competition-binding assay shows that RSV-3M3 targets antigenic site IV. Mutagenesis experiments have revealed RSV-3M3 requires arginine residue R429 for binding and neutralization. EM reconstruction shows that RSV-3M3 binds close to a 0° tilt. RSV-3M3 does not neutralize hMPV.
Antigen Distribution
F protein is a surface glycoprotein
Background
Respiratory syncytial virus (RSV) is a common respiratory virus that infects the majority of children by two years old1, 2. While usually mild, RSV can be serious in infants and older adults and is the leading cause of bronchiolitis and pneumonia in children less than one year of age in the United States1. A related pneumovirus, human metapneumovirus (hMPV), also significantly contributes to hospitalizations resulting from lower respiratory tract infection2. Antibodies have been described that bind and neutralize both RSV and hMPV fusion (F) proteins.

RSV F protein is a type I integral membrane protein that is synthesized as a 574 amino acid inactive precursor, assembled into a trimer, post-translationally modified, then cleaved to produce F1, F2, and intervening peptide pep273. Functional F protein has both pre- and post-fusion conformations. RSV F protein is highly conserved among RSV isolates from both A and B subgroups3 and is the primary target for antiviral drug development3 with several antigenic regions capable of introducing neutralizing antibodies2. RSV and hMPV F protein share ~36% sequence similarity.

Antigen Details

Protein
PubMed
Research Area
Infectious Disease
.
Respiratory Syncytial
.
Viral

References & Citations

1. National Center for Immunization and Respiratory Diseases (NCIRD), Division of Viral Diseases, Link Text
2. Mousa JJ, Binshtein E, Human S, et al. PLoS Pathog. 14(2):e1006837. 2018.
3. McLellan JS, Ray WC, Peeples ME. Curr Top Microbiol Immunol. 372:83-104. 2013.
Elisa Sandwich Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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