Anti-Rift Valley Fever Virus, Glycoprotein (Gn) (Clone RVFV-436) – Purified No Carrier Protein

Anti-Rift Valley Fever Virus, Glycoprotein (Gn) (Clone RVFV-436) – Purified No Carrier Protein

Product No.: R232

- -
- -
Product No.R232
Clone
RVFV-436
Target
Glycoprotein (Gn)
Rift Valley Fever Virus
Product Type
Recombinant Monoclonal Antibody
Alternate Names
RVFV, Gn, Glycoprotein
Isotype
Human IgG1
Applications
ELISA
,
N

- -
- -
Select Product Size
- -
- -

Antibody Details

Product Details

Reactive Species
Rift Valley Fever Virus
Expression Host
HEK-293 Cells
Immunogen
Sequenced from circulating B-cells isolated from a donor who was naturally infected with with RVFV.
Product Concentration
≥1.0 mg/ml
Purity
≥90% monomer by analytical SEC and SDS-Page
Formulation
This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
State of Matter
Liquid
Product Preparation
Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Antibodies may be stored sterile as received at 2-8°C for up to one year. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≥ -80°C. Avoid Repeated Freeze Thaw Cycles.
Regulatory Status
Research Use Only
Country of Origin
USA
Shipping
2 – 8° C Wet Ice
Additional Applications Reported In Literature ?
ELISA,
N
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Clone RVFV-436 binds the Gn glycoprotein of Rift Valley Fever Virus (RVFV).
Background
RVFV is a mosquito-borne phlebovirus primarily found in sub-Saharan Africa, especially in eastern and southern Africa. It infects both humans and ungulates (such as cows, goats, and sheep). RVFV causes a wide range of health effects, from mild illness to severe conditions like hemorrhagic disease, encephalitis, hepatitis, kidney injury, and retinitis. The virus can lead to spontaneous abortions in animals. RVFV outbreaks often occur during years of unusually heavy rainfall and flooding, as mosquitoes hatch more eggs, increasing transmission potential1,2.

RVFV-426 is a notable monoclonal antibody (mAb) that targets the Gn protein of the virus. This antibody exhibited significant neutralizing activity, with an IC50 value of 4.6 ng/mL for both the ZH501 and SA51 wild-type strains and 2.5 ng/mL for the MP-12 vaccine strain. The research underscores the potential of these mAbs in both prophylactic and therapeutic settings, offering a promising avenue for combating RVFV infections. The findings suggest that RVFV-436, along with other mAbs, could be further developed for use in humans to prevent or treat RVFV, which poses a significant threat to both human and animal health3.
Antigen Distribution
RVFV is primarily found in hepatic cells, endothelial cells, and mononuclear phagocytes in the human body. This distribution reflects the virus's tendency to cause hepatitis, hemorrhagic fever, and encephalitis during infection.
Research Area
Category A Pathogens
.
Infectious Disease
.
Viral
.
IVD Raw Material

References & Citations

1. Makoschey B, Kilsdonk E van, Hubers WR, et al. PLOS Neglected Tropical Diseases. 2016;10(3):e0004550.
2. Wandera N, Olds P, Muhindo R, Ivers L. N Engl J Med. 2023;389(20):1829-1832.
3. Chapman NS, Kose N, et al. PNAS.2021;118(14).
4. Jäckel S, Eiden M, Dauber M, Balkema-Buschmann A, Brun A, Groschup MH. Arch Virol. 2014;159(3):535-546.
Indirect Elisa Protocol
N

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.