A panel of human mAbs, including clone RRV-12, were sequenced from the peripheral blood mononuclear cells of two donors who were naturally infected with RRV1.
≥ 5.0 mg/ml
≤ 1.0 EU/mg as determined by the LAL method
≥95% monomer by analytical SEC
This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one year. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≥ -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Standard Overnight on Blue Ice.
Other Applications Reported In Literature ?
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
RRV-12 activity is directed against the E2 protein.
RoE2 is expressed on the surface of RRV.
Ross River Virus (RRV) is a mosquito-borne, positive sense, single-stranded virus endemic to Australia and Papua New Guinea that belongs to the arthritogenic group of alphaviruses1. The mature glycoprotein is composed of E1 and E2 envelope proteins in a heterodimer, expressed as a trimeric spike on the virus surface 2.
B cells were transformed with Epstein-Barr virus and then screened by direct virus binding ELISA. Hybridoma lines were established and mAbs were purified by single-cell flow cytometric sorting.
Clone RRV-12 is of the IgG1 isotype and is capable of binding and incompletely neutralizing prototype strain RRV T48 in a focus reduction neutralization test1.
References & Citations
1. Powell LA, Fox JM, Kose N, et al. PLoS Pathog. 16(5):e1008517. 2020.
2. Snyder AJ, Mukhopadhyay S. J Virol. 86(24):13609-20. 2012.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.