Anti-Ross River Virus (Clone RRV-12) – Purified No Carrier Protein

A panel of human mAbs, including clone RRV-12, were sequenced from the peripheral blood mononuclear cells of two donors who were naturally infected with RRV¹.

This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Standard Overnight on Blue Ice.

ELISA
N

RRV-12 activity is directed against the E2 protein.

Ross River Virus (RRV) is a mosquito-borne, positive sense, single-stranded virus endemic to Australia and Papua New Guinea that belongs to the arthritogenic group of alphaviruses¹. The mature glycoprotein is composed of E1 and E2 envelope proteins in a heterodimer, expressed as a trimeric spike on the virus surface ².

B cells were transformed with Epstein-Barr virus and then screened by direct virus binding ELISA. Hybridoma lines were established and mAbs were purified by single-cell flow cytometric sorting.

Clone RRV-12 is of the IgG1 isotype and is capable of binding and incompletely neutralizing prototype strain RRV T48 in a focus reduction neutralization test¹.

RoE2 is expressed on the surface of RRV.

1. Powell LA, Fox JM, Kose N, et al. PLoS Pathog. 16(5):e1008517. 2020.
2. Snyder AJ, Mukhopadhyay S. J Virol. 86(24):13609-20. 2012.