Anti-Ross River Virus (Clone: RRV-12)

Anti-Ross River Virus (Clone: RRV-12)

Product No.: LT574

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Product No.LT574
Clone
RRV-12
Target
Ross River Virus
Product Type
Recombinant Monoclonal Antibody
Alternate Names
RRV
Isotype
Human IgG1
Applications
ELISA
,
N

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Product Size
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Antibody Details

Product Details

Reactive Species
Ross River
Virus
Expression Host
HEK-293 Cells
Immunogen
A panel of human mAbs, including clone RRV-12, were sequenced from the peripheral blood mononuclear cells of two donors who were naturally infected with RRV1.
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
Formulation
This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one year. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≥ -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Standard Overnight on Blue Ice.
Additional Applications Reported In Literature ?
ELISA
N
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
RRV-12 activity is directed against the E2 protein.
Antigen Distribution
RoE2 is expressed on the surface of RRV.
Background
Ross River Virus (RRV) is a mosquito-borne, positive sense, single-stranded virus endemic to Australia and Papua New Guinea that belongs to the arthritogenic group of alphaviruses1. The mature glycoprotein is composed of E1 and E2 envelope proteins in a heterodimer, expressed as a trimeric spike on the virus surface 2.

B cells were transformed with Epstein-Barr virus and then screened by direct virus binding ELISA. Hybridoma lines were established and mAbs were purified by single-cell flow cytometric sorting.

Clone RRV-12 is of the IgG1 isotype and is capable of binding and incompletely neutralizing prototype strain RRV T48 in a focus reduction neutralization test1.

Antigen Details

Research Area
Infectious Disease
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Ross River
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Viral

References & Citations

1. Powell LA, Fox JM, Kose N, et al. PLoS Pathog. 16(5):e1008517. 2020.
2. Snyder AJ, Mukhopadhyay S. J Virol. 86(24):13609-20. 2012.
Indirect Elisa Protocol
N
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Formats Available

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Prod No.
Description
LT574
Products are for research use only. Not for use in diagnostic or therapeutic procedures.