Anti-Ross River Virus (Clone: RRV-19)

Pricing & Details

Product No.LT572
Clone
RRV-19
Protein
Ross River Virus
Formats AvailableView All
Product Type
Recombinant Monoclonal Antibody
Alternate Names
RRV
Isotype
Human IgG1
Applications
ELISA
,
N
Prod No.
Size
Price
Avail.
Qty
Add to cart
LT572-250 µg
250 µg
$390.00
Pre-order
Max:
Min: 1
Step: 1
LT572-1.0 mg
1.0 mg
$1,400.00
Pre-order
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Ross River
Virus
Expression Host
HEK-293
Immunogen
A panel of human mAbs were sequenced, including RRV-19, was generated from the peripheral blood mononuclear cells of two donors who were naturally infected with RRV1.
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
Formulation
This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one year. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≥ -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Standard Overnight on Blue Ice.
Other Applications Reported In Literature ?
ELISA
N
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
RRV-19 activity is directed against the B domain of the E2 protein and was determined by alanine scanning mutagenesis using cell-surface expression of RRV proteins and flow cytometric screening were used to identify critical binding residues in the E2 glycoprotein. Loss of binding occurs when residue 221 on the B domain of the E2 protein is mutated. Furthermore, clone RRV-19 is capable of neutralizing virus in both pre- and post-attachment neutralization assays.
Antigen Distribution
Ross River Virus E2 is expressed on the surface of RRV.
Background
Ross River Virus (RRV) is a mosquito-borne, positive sense, single-stranded virus endemic to Australia and Papua New Guinea that belongs to the arthritogenic group of alphaviruses1. The mature glycoprotein is composed of E1 and E2 envelope proteins in a heterodimer, expressed as a trimeric spike on the virus surface 2.

RRV-19 is of the IgG1 isotype and is capable of binding and neutralizing prototype strain RRV T48 as well as clinical isolate strains PW7, SN11, PW14, 2897601, and O’Regan in focus reduction neutralization tests1.

Antigen Details

Research Area
Infectious Disease
.
Ross River
.
Viral

References & Citations

1. Powell LA, Fox JM, Kose N, et al. PLoS Pathog. 16(5):e1008517. 2020.
2. Snyder AJ, Mukhopadhyay S. J Virol. 86(24):13609-20. 2012.
Elisa Sandwich Protocol

Formats Available

Prod No.
Description
LT572
Products are for research use only. Not for use in diagnostic or therapeutic procedures.