Anti-Ski Antibody (34021)
Anti-Ski Antibody (34021)
Product No.: 34021
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Clone G8 Target Ski Formats AvailableView All Product Type Monoclonal Alternate Names Proto-oncogene c-Ski Isotype Mouse IgG1 Applications IF , IP , WB |
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Antibody DetailsProduct DetailsReactive Species Human, Mouse, Rat, Chicken, Xenopus Host Species Mouse Immunogen Recombinant v-ski Product Concentration Lot Specific Formulation This monoclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.2 - 7.4 with no carrier protein or preservatives added. State of Matter Liquid Product Preparation Antibodies are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Upon initial thawing, appropriately aliquot and store at -80°C. For long-term storage, keep at -80°C. Avoid repeated freeze-thaw cycles. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1-5 ug/ml. Predicted molecular weight of v-ski is 55 kDa; full-length ski ranges from ~95 kDa up to ~115 kDa for hyperphosphorylated ski
Immunocytochemistry: use at 1-10 ug/ml. ski is a nuclear protein. Immunoprecipitation: use at 1-5 ug/ml. Positive controls: Recombinant cell lines expressing ski proteins. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This antibody recognizes ski protein of human, mouse, rat, chicken, and Xenopus. Background The ski oncogene family (ski and sno) encodes transcriptional regulators that can affect oncogenic transformation and cellular differentiation. ski and sno function as coactivators or co-repressors, modulating transcription by interacting with transcription factor complexes. ski interacts with members of the Nuclear Factor I (NFI) family, the retinoic acid receptor, and the nuclear hormone co-regulators NCoR and NCoA62/skip. Function May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling. {PubMed:19049980}. NCBI Gene Bank ID UniProt.org Research Area Cancer Research References & CitationsTechnical ProtocolsIF   Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
