This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This purified antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Anti-Syntaxin (Clone HPC-1)recognizes a (Mr 35 kD) polypeptide on the cell membrane of amacrine cells.1 In hippocampus, this antibody recognizes the stratum radiatum, the layer formed by the fibers of dentate granule cells. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Syntaxins (syntaxin isotypes 1-4) are membrane integrated Q-SNARE proteins participating in exocytosis. Syntaxins bind synaptotagmin in a calcium-dependent fashion and interact with voltage dependent calcium and potassium channels via the C-terminal H3 domain. Direct syntaxin-channel interaction is a suitable molecular mechanism for proximity between the fusion machinery and the gates of Ca2 + entry during depolarization of the presynaptic axonal boutons.
References & Citations
1. Barnstable, C. J. et al. (1985) Dev. Brain Res. 20:286 2. Inoue, A. et al. (1992) J. Biol. Chem. 267:10613
Products are for research use only. Not for use in diagnostic or therapeutic procedures.