Anti-TARPγ2/4/8Stargazin Antibody (11572)
Anti-TARPγ2/4/8Stargazin Antibody (11572)
Product No.: 11572
- -
- -
Clone S245-36 Target TARPγ2/4/8Stargazin Formats AvailableView All Product Type Monoclonal Alternate Names Neuronal voltage-gated calcium channel γ-2 subunit, Stargazin, Transmembrane AMPAR regulatory protein γ-2, TARP γ-2 Isotype Mouse IgG2a Applications ICC , IF , WB |
Data
Western Blot analysis of Rat brain lysates showing detection of Stargazin Calcium Channel protein using Mouse Anti-Stargazin Calcium Channel Monoclonal Antibody, Clone S245-36 (11572). Primary Antibody: Mouse Anti-Stargazin Calcium Channel Monoclonal Antibody (11572) at 1:100, 1:250, 1:500, and 1:1000.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody, Clone S245-36 (11572). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody (11572) at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TARP Gamma2/4/8 (Stargazin) Antibody (D) Composite.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody, Clone S245-36 (11572). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody (11572) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) TARP Gamma2/4/8 (Stargazin) Antibody. (D) Composite. - -
- -
Antibody DetailsProduct DetailsReactivity Species Mouse ⋅ Rat Host Species Mouse Immunogen Fusion protein corresponding to aa 203-323 (cytoplasmic C-terminus) of rat TARPg2 (accession no. Q71RJ2). Product Concentration Lot Specific Formulation PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Avoid repeated freeze-thaw cycles. Regulatory Status For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1ug/mL. Predicted molecular weight is 35-55kDa.
Positive control: Rat brain lysate. These are recommended concentrations. User should determine optimal concentrations for their application. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This antibody recognizes mouse and rat TARPg2, TARPg4, and TARPg8. It does not cross-react with TARPg3 Background Stargazin belongs to a group of voltage- gated calcium channels that are present in most excitable cells. It appears to stabilize calcium channels in an inactivated (closed) state. Voltage-gated calcium channels exert spatial and temporal control over cellular calcium concentrations and serve to modulate neurotransmitter release, hormone secretion, muscle contraction, electrical activity, cell metabolism and proliferation, gene expression, and neuronal survival. Antigen DetailsFunction Regulates the trafficking and gating properties of AMPA-selective glutamate receptors (AMPARs). Promotes their targeting to the cell membrane and synapses and modulates their gating properties by slowing their rates of activation, deactivation and desensitization. Does not show subunit-specific AMPA receptor regulation and regulates all AMPAR subunits. Thought to stabilize the calcium channel in an inactivated (closed) state. {PubMed:17880894, PubMed:18817736, PubMed:19234459, PubMed:19265014, PubMed:20805473}. NCBI Gene Bank ID UniProt.org Research Area Ion Channels References & CitationsTechnical ProtocolsICC IF  |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
