Anti-TGF-β3 (Clone 20724)
Anti-TGF-β3 (Clone 20724)
Product No.: T431
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Clone 20724 Target TGF-beta 3 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names TGFB3, ARVD, FLJ16571, Transforming Growth Factor β3 Isotype IgG1 Applications IHC FFPE , N , WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Mouse Immunogen Purified Recombinant Mouse TGF-β3 (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831931 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse TGF-β3 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Mouse TGF-β3 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T198. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 25 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Neutralization: This antibody is useful for neutralization of Mouse TGF-β3 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse TGF-β3 (at 0.1 ng/ml) is 0.1-0.3 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Mouse Transforming Growth Factor β3 (TGF-β3) (Clone 20724) recognizes an epitope on Mouse TGF-β3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows less than 25% cross-reactivity with rhTGF-β1.2 and rhTGF-β2, and less than 2% cross-reactivity with raTGF-β5 and rhTGF-β1. Background Transforming growth factor-beta 3 (TGF-beta 3) is a member of the TGF beta family of growth factors. It is involved in oxygen-dependent differentiation processes during placental development and pregnancy disorders.1 It also play an important role in wound repair and scarring.2 Antigen DetailsPubMed References & Citations1. Mustoe, TA. et al. (1997) Arch Surg. 132: 753 2. Wenger, RH. et al. (2003) Placenta 24: 941 Technical ProtocolsIHC FFPE N  |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
