Name: Anti-TSG101 [Clone 4A10]
SKU: 3352
Price: 675.00 USD
Availability: InStock

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Anti-TSG101 [Clone 4A10]

Product No.: 3352


Clone 4A10 Target TSG101 Formats AvailableView All Product Type Monoclonal Alternate Names ESCRT-I complex subunit TSG101 Isotype Mouse IgG1 Applications ELISA , EM , FACS , ICC , IF , IHC , IHC FFPE , IP , WB


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Data

TSG101 antibody [4A10] detects TSG101 protein by western blot analysis. A. 30 ug NIH-3T3 whole cell lysate/extract B. 30 ug JC whole cell lysate/extract C. 30 ug BCL-1 whole cell lysate/extract 10% SDS-PAGE TSG101 antibody [4A10] (3352) dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

ELISA detection of TSG101 using for capture at a concentration of 5 ug/mL and 3352 for detection at a concentration of 1.5 ug/mL.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (3352) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. TSG101 stained by TSG101 antibody [4A10] (3352) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer. TSG101 stained by TSG101 antibody [4A10] (3352) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

TSG101 antibody [4A10] (3352) detects TSG101 protein by flow cytometry analysis. Sample: THP-1 cell. Black: Unlabelled sample was used as a control. Red: TSG101 antibody [4A10] (3352) dilution: 1:25. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (3352) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.

Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (3352) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. TSG101 stained by TSG101 antibody [4A10] (3352) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Antibody Details

Product Details

Reactive Species

Hamster

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Human

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Monkey

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Mouse

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Rat

Host Species

Mouse

Immunogen

Recombinant protein corresponding to aa 167-374 of TSG101 protein

Product Concentration

Lot Specific

Formulation

PBS, pH 7.2.

State of Matter

Liquid

Product Preparation

Purified by affinity chromatography

Storage and Handling

This product is stable for at least one (1) year if stored at -20°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

Regulatory Status

Research Use Only

Country of Origin

USA

Shipping

Next Day 2-8°C

Applications and Recommended Usage?
Quality Tested by Leinco

ELISA: use at 1-5ug/ml.

Immunoblotting: use at a 1:500-1:3,000 dilution. A band of 46kDa is detected. Detection of TSG101 protein in (A) NIH-3T3 cell lysate, (B) JC cell lysate, and (C) BCL-1 cell lysate with #3352 diluted 1:500.

Endusers should determine optimal antibody concentrations for their applications.

IHC: use at a dilution of 1:100 with antigen retrieval: citrate buffer, pH 6.0, 15 minutes. Detection of TSG101 protein in paraffin-embedded human breast carcinoma. Detection of TSG101 protein in paraffin-embedded human ovarian carcinoma.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

This antibody recognizes human, mouse, rat, hamster, and monkey TSG101 protein.

Background

Tumor Susceptibility Gene 101 (TSG101) codes for a 46kDa protein whose inactivation in mouse fibroblasts results in cell transformation and tumor formation in nude mice. Mutations in TSG101 have been linked to cervical, breast, prostate and gastrointestinal cancers. In addition, TSG101 is one of the cellular proteins involved in the budding process of HIV-1, and plays an important role in the cellular vacuolar protein sorting (Vps) pathway. Its main function being recognizing ubiquitinated cargo, TSG101 also proved to be essential for the budding process of HIV-1 virions.

Function

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). It may also play a role in the extracellular release of microvesicles that differ from the exosomes (PubMed:22315426). {PubMed:11916981, PubMed:17556548, PubMed:17853893, PubMed:21070952, PubMed:21757351, PubMed:22315426, PubMed:22660413}.

NCBI Gene Bank ID

TSG101

UniProt.org

Q99816

Research Area

Cancer Research