Anti-trimethyl-Histone H3 (Lys9) Antibody, clone 6F12-H4 – Purified No Carrier Protein

This monoclonal antibody was raised against a synthetic peptide from Human Histone H3, tri-methylated at Lysine-9 [UniProt# P84243].

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Liquid

Purified antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

2 – 8° C Wet Ice

6F12-H4 activity is directed against human and rat trimethylated H3 (Lys9), H3K9me3.

Histones are highly conserved nuclear proteins that form the structural scaffold for organizing genomic DNA into chromatin. The nucleosome—the fundamental subunit of chromatin—consists of 146 base pairs of DNA wrapped around a core histone octamer containing two molecules each of H2A, H2B, H3, and H4. Post-translational modifications (PTMs) of these histone tails, such as acetylation, phosphorylation, ubiquitination, and methylation, dynamically regulate DNA transcription, repair, recombination, and replication by altering chromatin architecture or recruiting specific reader proteins (the "histone code" hypothesis).

Trimethylation of Histone H3 at Lysine 9 (H3K9me3) is a critical, highly studied epigenetic mark predominantly associated with transcriptional repression and heterochromatin assembly. H3K9me3 acts as a specific binding motif that recruits chromatin-associated silencing proteins, including HP1α/β and Swi6. The generation of this repressive mark is primarily catalyzed by the histone methyltransferases SUV39H1 and SUV39H2.

6F12-H4 was generated against a KLH-conjugated, branched synthetic peptide, (QTARme3K- STGGKA)2-KC.

H3 is expressed in nucleosomes.

1 Scott WA, Campos EI. Front Cell Dev Biol. 8:701. 2020.
2 Gil L, Chi-Ahumada E, Niño SA, et al. Int J Mol Sci. 23(21):12873. 2022.
3 Batugedara G, Lu XM, Hristov B, et al. Nat Commun. 14(1):5086. 2023.
4 Gaskill MM, Soluri IV, Branks AE, et al. Dev Cell. 58(17):1610-1624.e8. 2023.
5 Ma P, An T, Zhu L, et al. Development. 147(21):dev188078. 2020.