Human NT-proBNP

N-terminal pro-brain natriuretic peptide (NT-proBNP) is widely used as a diagnostic biomarker for heart failure and cardiac dysfunction in both clinical and forensic settings¹. NT-proBNP is found in plasma¹ and urine² after being synthesized and secreted by myocytes in the left ventricle in response to pressure overload or ventricular volume expansion¹.

Mechanical stress induces the BNP promoter via p38 MAPK signaling, and a 134 aa BNP precursor (pre-proBNP) is rapidly translated from the unstable RNA¹. Subsequently, proBNP is formed by removing a 26 aa N-terminal signal peptide. ProBNP is then split into the 76 aa NT- proBNP and 32 aa BNP, which has diuretic, natriuretic, and vasodilatory effects. BNP and NT- proBNP have a half-life of ~20 and 90-120 minutes, respectively. Additionally, NT-proBNP remains elevated for ~12 weeks post myocardial infarction.

BNP and NT-proBNP are used as biomarkers to diagnose the severity of heart failure and cardiac dysfunction as well as to guide treatment strategies¹. The upper limit for normal in the non-acute setting is 35 pg/mL for BNP and 125 pg/mL for NT-proBNP, and 100 pg/mL and 300 pg/mL in the acute setting, respectively. Additionally, age-dependent cutoffs for NT-proBNP can be used to diagnose heart failure (patients <50 years with levels >450 pg/mL; 50-75 years with levels >900 pg/mL; >75 years with levels >1800 pg/mL).

NT-proBNP is also related to arrhythmias and cardiomyopathies and is increased in atrial fibrillation patients¹. Additionally, there is a positive correlation between NT-proBNP levels and the risk of death, and a reduction in NT-proBNP levels predicts an improvement in clinical symptoms. As such, NP-proBNP is a useful diagnostic indicator to manage patient treatment. NP-proBNP is also useful in forensic pathology to clarify the mechanism of death; NT-proBNP is stable for at least 24 days postmortem.

Human

P16860

HPLGSPGSAS DLETSGLQEQ RNHLQGKLSE LQVEQTSLEP LQESPRPTGV WKSREVATEG IRGHRKMVLY TLRAPR

Liquid

~10kDa

~9.5kDa

1 month, 2 to 8 °C under sterile conditions after opening. 1 year from date of receipt, -20 to -80 °C as supplied. 3 months from date of receipt, -20 to -80 °C sterile conditions after opening

Frozen Dry Ice

Human

Research Use Only

1 Cao Z, Jia Y, Zhu B. Int J Mol Sci. 20(8):1820. 2019.
2 Müller N, Bayer E, Bernhardt C, et al. Clin Chim Acta. 529:4-9. 2022.
3 Seferian KR, Tamm NN, Semenov AG, et al. Clin Chem. 54(5):866-873. 2008.
4 Chen YY, Li SL, Lin HL, et al. Anal Biochem. 611:113950. 2020.
5 Srisujikul P, Thiankhaw K, Tanprawate S, et al. Sci Rep. 13(1):13903. 2023.
6 Colom G, Salvador JP, Acosta G, et al. Analyst. 145(20):6719-6727. 2020.