Influenza H5 (A/bovine/Ohio/B24OSU-439/2024) – Purified No Carrier Protein

The H5 subtype is a member of the Group 1 influenza hemagglutinins and is a hallmark of Highly Pathogenic Avian Influenza (HPAI)^4,6. Because H5 viruses possess a polybasic cleavage site in the HA protein, they can be processed by a wide range of ubiquitous proteases, leading to systemic infection and high mortality rates in avian hosts^2,5.

Recombinant H5 protein is a critical reagent for the development of subunit vaccines, the generation of subtype-specific monoclonal antibodies, and the standardization of diagnostic assays such as the Hemagglutination Inhibition (HI) test^1,4,6. It is also used in structural biology to map the antigenic evolution of the virus and to identify conserved "stalk" epitopes that are targets for universal influenza vaccine strategies^3,5.

Influenza A Virus (H5N1)

Verified by Hemagglutination with Erythrocytes

Q207Z6

MEKIVLLLAIVSLVKSDQICIGYHANNSTEQVDTIMEKNVTVTHAQDILEKTHNGKLCDLDGVKPLILRDCSVAGWLLGNPMCDEFLNVPEWSYIVEKINPANDLCYPGNFNDYEELKHLLSRINHFEKIQIIPKSSWSDHEASAGVSSACPYQGRSSFFRNVVWLIKKDNAYPTIKRSYNNTNQEDLLVLWGIHHPNDAAEQTRLYQNPTTYISVGTSTLNQRLVPKIATRSKVNGQSGRMEFFWTILKPNDAINFESNGNFIAPENAYKIVKKGDSTIMKSELEYGNCNTKCQTPIGAINSSMPFHNIHPLTIGECPKYVKSSRLVLATGLRNSPQGERRRKKRGLFGAIAGFIEGGWQGMVDGWYGYHHSNEQGSGYAADKESTQKAIDGVTNKVNSIIDKMNTQFEAVGREFNNLERRIENLNKKMEDGFLDVWTYNAELLVLMENERTLDFHDSNVKNLYDKVRLQLRDNAKELGNGCFEFYHRCDNECMESVRNGTYDYPQYSEEARLKREEISGVKLESIGTYQILSIYSTVASSLALAIMVAGLSLWMCSNGSLQCRICI

Lyophilized

~40 kDa (HA1); ~25 kDa (HA2)

~75 kDa (HA0 full length)

Reconstitute at 0.1-1 mg/ml using filtered deionized water. Gently mix by vortexing and/or inversion until fully dissolved. Centrifuge if necessary.

This lyophilized protein is stable for twelve months when stored at -20°C to -70°C. After aseptic reconstitution, this protein may be stored for one month at 2°C to 8°C or for three months at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Frozen Dry Ice

alpha-2,3-linked Sialic Acid Receptors

Influenza A Virus (H5N1)

Research Use Only

1 Skehel JJ, Wiley DC. Receptor binding and membrane fusion in virus entry: the influenza hemagglutinin. Annu Rev Biochem. 69:531-69. 2000.
2 Neumann G, Noda T, Kawaoka Y. Emergence and pandemic potential of swine-origin H1N1 influenza virus. Nature. 459(7249):931-9. 2009.
3 Gamblin SJ, et al. The structure and receptor binding of the hemagglutinin from a human H5N1 influenza virus. Science. 303(5665):1838-42. 2004.
4 Lu JH, et al. A mouse monoclonal antibody that neutralizes several strains of highly pathogenic H5N1 influenza virus. J Virol. 80(22):10981-8. 2006.
5 Wiley DC, Skehel JJ. The structure and function of the hemagglutinin membrane glycoprotein of influenza virus. Annu Rev Biochem. 56:365-94. 1987.
6 World Health Organization. Manual for the laboratory diagnosis and virological surveillance of influenza. WHO Press. 2011.