Anti-Free-Beta (β) HCG [Clone 148-HEK] – Purified

This purified antibody is supplied in 0.05 M phosphate buffered saline (PBS), pH 7.2 - 7.4, containing 0.1% sodium azide. as a preservative.

Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

The HEK293 Expressed Advantage To guarantee optimal functional activity, this monoclonal antibody is recombinantly expressed and HEK Purified. Production in Human Embryonic Kidney (HEK) mammalian cell lines ensures that the antibody undergoes proper human-like post-translational modifications, including accurate protein folding and complex glycosylation. Because it is recombinantly produced rather than relying on traditional hybridoma ascites, the HEK expression system provides exceptional lot-to-lot consistency, high purity, and scalability. Free from animal-derived serum contaminants, the HEK-purified Clone 148-HEK delivers the rigorous reproducibility required for both advanced translational research and commercial diagnostic manufacturing.

To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s recombinant biosimilar antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

Research Use Only

anti-free beta HCG

Biological Significance of Free Beta hCG

Human Chorionic Gonadotropin (hCG) is a vital glycoprotein hormone primarily responsible for the establishment and maintenance of pregnancy. Structurally, intact hCG is a heterodimer composed of two non-covalently linked subunits: an alpha subunit (shared with other glycoprotein hormones like LH, FSH, and TSH) and a distinct beta subunit (β-hCG) that confers unique biological specificity. While most hCG circulates in its intact form, a fraction of the beta subunit circulates independently as Free Beta hCG.

Measuring Free Beta hCG has become a critical tool in clinical diagnostics. It is heavily utilized in obstetrics and gynecology as a primary biomarker for first-trimester prenatal screening—most notably in assessing the risk for Trisomy 21 (Down Syndrome) and evaluating ectopic pregnancies. Additionally, Free Beta hCG serves as an important oncological tumor marker for tracking trophoblastic diseases, choriocarcinoma, and testicular germ cell tumors.

High Specificity with Clone 148-HEK

The Anti-Free Beta hCG Monoclonal Antibody (Clone 148-HEK) is engineered to specifically target the uncombined, free circulating beta subunit of the hCG molecule. By recognizing an epitope uniquely accessible on the free beta subunit, Clone 148-HEK effectively differentiates between free β-hCG and the intact heterodimeric hCG hormone. This exceptional specificity this monoclonal antibody an ideal reagent for developing highly sensitive In Vitro Diagnostic (IVD) assays, quantitative ELISAs, and lateral flow assays (LFAs), ensuring accurate results with minimal to no cross-reactivity with intact hCG, LH, FSH, or TSH.

1.) Canick JA, Kellner LH. First trimester screening for aneuploidy: serum biochemical markers. Semin Perinatol. 1999 Oct;23(5):359-68. doi: 10.1016/s0146-0005(99)80002-0. PMID: 10551789.
2.) Katharine D Wenstrom, John Owen. Free β-hCG Subunit Versus Intact hCG in Down Syndrome Screening, Obstetrics & Gynecology, Volume 90, Issue 3, 1997, Pages 370-374,