Anti-Free-Beta (β) HCG [Clone 148-HEK] – Purified

This purified antibody is supplied in 0.05 M phosphate buffered saline (PBS), pH 7.2 - 7.4, containing 0.1% sodium azide. as a preservative.

Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

The HEK293 Expressed Advantage To guarantee optimal functional activity, this monoclonal antibody is recombinantly expressed and HEK Purified. Production in Human Embryonic Kidney (HEK) mammalian cell lines ensures that the antibody undergoes proper human-like post-translational modifications, including accurate protein folding and complex glycosylation. Because it is recombinantly produced rather than relying on traditional hybridoma ascites, the HEK expression system provides exceptional lot-to-lot consistency, high purity, and scalability. Free from animal-derived serum contaminants, the HEK-purified Clone 148-HEK delivers the rigorous reproducibility required for both advanced translational research and commercial diagnostic manufacturing.

To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s recombinant biosimilar antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

anti-free beta HCG

Human chorionic gonadotropin (hCG) is comprised of two subunits: 1) the alpha subunit (14.5 kDa), which is also found in LH, FSH, and TSH, and 2) the beta subunit (hCGβ, 22.2 kDa), which is unique to hCG. In normal physiology, hCG is produced by syncytiotrophoblast and cytotrophoblast cells in the placenta during pregnancy, where it promotes progesterone production and embryo implantation through interaction with its ligand LHCGR, among numerous other functions (1,2).

Free hCGβ is an alternatively glycosylated variant of hCG that acts as an LHCGR agonist and a TGFβ antagonist (2). Some research suggests a link between increased circulating hCGβ and gestational hypertension (3), as well as between hCGβ and Down’s syndrome (4). Further, free hCGβ promotes cancer cell growth and malignancy, with multiple studies showing a correlation between free hCGβ levels and poor prognosis (5).

Other variants of hCGβ have also been implicated in cancer progression. In pregnancy, hyperglycosylated hCG acts on cytotrophoblast cells via autocrine signaling to decrease apoptosis and promote embryo implantation/invasion (1,2). Choriocarcinomas and germ cell tumors therefore secrete large amounts of hyperglycosylated hCG to take advantage of these natural functions (3,6). hCG is also known to have immunomodulatory functions, essential during pregnancy to promote maternal tolerance of the embryo (2).

1. Cole L. A. (2010). Biological functions of hCG and hCG-related molecules. Reproductive biology and endocrinology : RB&E, 8, 102.

2. d’Hauterive, S. P., Close, R., Gridelet, V., Mawet, M., Nisolle, M., & Geenen, V. (2022). Human Chorionic Gonadotropin and Early Embryogenesis: Review. International Journal of Molecular Sciences, 23(3), 1380.

3. Wang, R., Chen, L., Wang, X., & Liu, Y. (2021). Association between serum beta-human chorionic gonadotropin and inflammation, oxidative stress in pregnancy-induced hypertension. Microvascular research, 135, 104130.

4. Ballantyne, A., Rashid, L., & Pattenden, R. (2022). Stability of maternal serum free β-hCG following whole blood sample transit: First trimester Down’s syndrome screening in Scotland. Annals of Clinical Biochemistry, 59(1), 87-91.

5. Iles, R. K. (2007). Ectopic hCGβ expression by epithelial cancer: malignant behaviour, metastasis and inhibition of tumor cell apoptosis. Molecular and cellular endocrinology, 260, 264-270.

6. Cole, L. A., & Butler, S. A. (2008). Hyperglycosylated human chorionic gonadotropin and human chorionic gonadotropin free beta-subunit: tumor markers and tumor promoters. The Journal of Reproductive Medicine, 53(7), 499-512.