Anti-Mouse CD7 [Clone JD23] — PE

This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.

PE‑conjugated anti‑mouse CD7 antibody was purified from tissue culture supernatant by affinity chromatography, then conjugated to R‑phycoerythrin (PE) using a controlled labeling procedure to preserve antigen‑binding activity and fluorophore performance. The final conjugate is supplied in a buffered aqueous solution containing stabilizing proteins and a preservative, and is filtered for use in flow cytometry applications requiring sensitive and specific detection of mCD7‑expressing cells.

This R-phycoerythrin (R-PE) conjugate is stable when stored at 2-8°C. Do not freeze.

Next Day 2-8°C

Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Functional Assay (FA): JD23 is a mouse CD7‑specific antibody that recognize cell‑surface mCD7; however, only CAR‑T cells engineered with the JD23‑derived scFv mediate potent and specific killing of mCD7‑positive tumor cells in vitro.

This antibody specifically recognizes mouse CD7 (mCD7) and shows no detectable cross‑reactivity with unrelated mouse surface markers under the tested conditions. Binding has been confirmed on mCD7‑expressing cells and is absent on mCD7‑negative control cells and isotype controls, supporting its suitability for specific detection of mouse CD7 in immunological and CAR‑T–related applications.

CD7, also known as Leu‑9, is an approximately 40 kD glycosylated and palmitoylated transmembrane protein belonging to the immunoglobulin superfamily. The extracellular domain of mature mouse CD7 shares 43% and 81% amino acid sequence similarity with human and rat CD7, respectively. Mouse CD7 mRNA has been detected in thymus, spleen, bone marrow, and small intestine, consistent with its predominant expression on T cells and NK cell subsets involved in immune regulation. Although CD7 has been implicated in T‑cell activation and signaling in other species, both the physiological ligand and precise function of mouse CD7 remain incompletely defined, making it a useful target and marker in T‑cell biology, leukemia/lymphoma research, and CAR‑T cell development.

Mouse CD7 mRNA exists in thymus, spleen, bone marrow, and small intestine

Hematopoietic stem and progenitors, Leukemia, NK cells, T cells, Thymocytes

1. Kim MY, Cooper ML, Jacobs MT, Ritchey JK, Hollaway J, Fehniger TA, DiPersio JF. CD7-deleted hematopoietic stem cells can restore immunity after CAR T cell therapy. JCI Insight. 2021 Aug 23;6(16):e149819. doi: 10.1172/jci.insight.149819. PMID: 34423790; PMCID: PMC8410010.