Anti-Mouse CD7 [Clone JD23] — Purified in vivo GOLD™ Functional Grade

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Our functional-grade preclinical antibodies are produced in a dedicated animal-free facility using advanced in vitro cell culture methods. To ensure peak performance and purity, we employ a multi-step purification protocol—including Protein A or G chromatography—to guarantee exceptionally low levels of endotoxins, protein aggregates, and leachable Protein A.

This purified monoclonal antibody is stable when stored at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Next Day 2-8°C

Functional Assay (FA): JD23 is a mouse CD7‑specific antibody that recognize cell‑surface mCD7; however, only CAR‑T cells engineered with the JD23‑derived scFv mediate potent and specific killing of mCD7‑positive tumor cells in vitro.

This antibody specifically recognizes mouse CD7 (mCD7) and shows no detectable cross‑reactivity with unrelated mouse surface markers under the tested conditions. Binding has been confirmed on mCD7‑expressing cells and is absent on mCD7‑negative control cells and isotype controls, supporting its suitability for specific detection of mouse CD7 in immunological and CAR‑T–related applications.

CD7 (Leu-9): A Key Marker in T-Cell Biology and CAR-T Research
CD7, also known as Leu-9, is an approximately 40 kD glycosylated and palmitoylated transmembrane protein belonging to the immunoglobulin superfamily. As a critical surface antigen, CD7 plays a significant role in immune regulation and oncology research.

Molecular Characteristics and Homology
The extracellular domain of mature mouse CD7 shares specific amino acid sequence similarities with other species, making it a vital focus for cross-species comparative studies:

81% similarity with rat CD7.

43% similarity with human CD7.

CD7 Expression and Localization
In mice, CD7 mRNA is predominantly detected in lymphoid and mucosal tissues, reflecting its role in the immune system. Key expression sites include:

-Thymus and Spleen
-Bone Marrow
- Small Intestine

This expression pattern is consistent with its presence on T cells and NK cell subsets, where it assists in various stages of immune signaling.

Research Applications: Leukemia and CAR-T Therapy
While the physiological ligand and exact function of mouse CD7 are still being defined, it remains a high-value biomarker and therapeutic target. Its primary applications include:

- T-cell activation and signaling research. - Leukemia and lymphoma diagnostics and monitoring.

Next-generation CAR-T cell development, specifically for targeting T-cell malignancies.

Mouse CD7 mRNA exists in thymus, spleen, bone marrow, and small intestine

Hematopoietic stem and progenitors, Leukemia, NK cells, T cells, Thymocytes

1. Kim MY, Cooper ML, Jacobs MT, Ritchey JK, Hollaway J, Fehniger TA, DiPersio JF. CD7-deleted hematopoietic stem cells can restore immunity after CAR T cell therapy. JCI Insight. 2021 Aug 23;6(16):e149819. doi: 10.1172/jci.insight.149819. PMID: 34423790; PMCID: PMC8410010.