This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This R-PE conjugate is stable when stored at 2-8°C. Do not freeze.
Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: 100%
Human IgG2: 100%
Human IgG3: 100%
Human IgG4: 100%
Human IgM: <0.2%
Human IgA: <0.1%
Human IgE: <0.1%
There is no detectable binding to bovine, goat, horse sheep IgG.
NOTE: This Anti-Human IgG, Fc Fragment Specific (Clone HP6043) when conjugated to HRPO is validated for use in Enzyme Immunoassay for the detection of Human IgG, Fc Fragment Specific (Part No.: I-1200
Country of Origin
Applications and Recommended Usage?
Quality Tested by Leinco
FC This antibody has been quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. Each investigator should determine their own optimal working dilution for specific applications.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Anti-Human IgG (Fc Specific) monoclonal antibody (clone HP6043) has been purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody reacts with all IgG isotypes and is non-reactive with IgA, IgM, IgE, light chains or F(ab')2 portions of human IgG.
Surface IgG is expressed on approximately 2% of normal human peripheral blood cells.
References & Citations
1. Reimer, C. B. et al. (1984) Hybridoma 3:263 2. Jefferies, R. et al. (1985) Immunol. Letters 10:223 3. Phillips, D. J. et al. (1987) Immunol. Letters 17:159
Products are for research use only. Not for use in diagnostic or therapeutic procedures.