Anti-Human IgG Fc Specific – HRP

Anti-Human IgG Fc Specific – HRP

Product No.: I-1200

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Product. No.I-1200
Clone
HP6043
Antibody Type
Monoclonal Antibody
Isotype
Mouse
IgG2b

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Product Size
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Product Concentration
0.5 mg/ml
Formulation
This HRP-conjugated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4, 1% BSA. <b> (Warning: Use of sodium azide as a preservative will inhibit the enzyme activity of horseradish peroxidase)</b>
Product Preparation
Clone HP6043, Anti-Human IgG Fc Specific monoclonal antibody has been purified using multi-step affinity chromatography methods using protein A.
Storage and Handling
This conjugated antibody is stable when stored at 2-8°C. Do not freeze.
Cross Reactivity
Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: 100%
Human IgG2: 100%
Human IgG3: 100%
Human IgG4: 100%
Human IgM: <0.2%
Human IgA: <0.1%
Human IgE: <0.1%

There is no detectable binding to bovine, goat, horse sheep IgG.

NOTE: This Anti-Human IgG, Fc Fragment Specific (Clone HP6043) when conjugated to HRPO is validated for use in Enzyme Immunoassay for the detection of Human IgG, Fc Fragment Specific (Part No.: I-1200)
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Enzyme Immunoassay 1:1000; see application references
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
This antibody reacts with all IgG isotypes and is non-reactive with IgA, IgM, IgE, light chains or F(ab')2 portions of human IgG.

Antigen Details

Ligand/Receptor
CD16, CD32, CD64

References & Citations

1. Reimer, C. B. et al. (1984) Hybridoma 3:263 2. Jefferies, R. et al. (1985) Immunol. Letters 10:223 3. Phillips, D. J. et al. (1987) Immunol. Letters 17:159
Indirect Elisa Protocol

Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.