Anti-Human IgG Fc Specific – Purified In vivo GOLD™ Functional Grade

Pricing & Details

Product No.I-1193
Clone
HP6043
Protein
Fc Fragment Specific
IgG
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
Mouse
IgG2b
Applications
ELISA
,
FC
Prod No.
Size
Price
Avail.
Qty
Add to cart
I-1193-0.25 mg
0.25 mg
$150.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Product Concentration
1.0 - 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE and HPLC
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
This antibody is stable for at least four (4) weeks when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Cross Reactivity
Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: 100%
Human IgG2: 100%
Human IgG3: 100%
Human IgG4: 100%
Human IgM: <0.2%
Human IgA: <0.1%
Human IgE: <0.1%

There is no detectable binding to bovine, goat, horse sheep IgG.

NOTE: This Anti-Human IgG, Fc Fragment Specific (Clone HP6043) when conjugated to HRPO is validated for use in Enzyme Immunoassay for the detection of Human IgG, Fc Fragment Specific (Part No.: I-1200)
Country of Origin
USA
Applications and Recommended Usage?
Quality Tested by Leinco
FC This antibody has been quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.
ELISA This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human IgG, Fc Fragment Specific (Clone HP6043) recognizes Human IgG, Fc Fragments. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Details

Elisa Sandwich Protocol
Flow Cytometry

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.