Recombinant Human CXCL14

Chemokine (C-X-C motif) ligand 14 (CXCL14) is a small cytokine belonging to the CXC chemokine family that is also known as BRAK (for breast and kidney-expressed chemokine).¹ CXCL14 is known to be a chemoattractant for monocyte and dendritic cells.² CXCL14 might play a pivotal role in the pathobiology of pancreatic cancer, probably by regulating cancer invasion.³

The biological activity of Human BRAK was determined by its ability to induce calcium flux of prostaglandin E2 treated THP-1 cells (Kurth, I. et al., 2001, J. Exp. Med. 194:855). 0.25 μg/mL of rhCXCL14 can significantly induce calcium flux.

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Ser35

Solution

The predicted molecular weight of Recombinant Human CXCL14 is Mr 9.4 kDa.

9.4

This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 with no calcium, magnesium, or preservatives present.

Working aliquots of this recombinant protein solution are stable for up to twelve months at -20°C to -70°C in a manual defrost freezer. Upon thawing, in the presence of a carrier protein, this recombinant protein can be stored at 2° - 8°C for one month without detectable loss of activity. For long-term storage, aliquot and freeze at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles.

Dry Ice

1. Moser, B. et al. (2004) Cytokine 44: 248
2. Chihara, K. et al. (2007) Biochem Biophys Res Commun. 364: 1037
3. Friess, H. et al. (2008) Cancer Lett. 259: 209