Anti-Human CXCL14 (Clone 131120)
Pricing & Details
E. coli-Derived Recombinant Human CXCL14 (Accession # O95715)
<0.1 EU/µg as determined by the LAL method
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human CXCL14 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CXCL14 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Other Applications Reported In Literature ?
ELISA Capture: Suitable for use at concentration of 2-8 µg/mL. Use in combination with: Detection Reagent: Human CXCL14/BRAK Biotinylated Antibody. Standard: Recombinant Human CXCL14/BRAK Protein, CF.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Mouse Anti-Human CXCL14 (Clone 131120) recognizes an epitope on Human CXCL14. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Chemokine (C-X-C motif) ligand 14 (CXCL14) is a small cytokine belonging to the CXC chemokine family that is also known as BRAK (for breast and kidney-expressed chemokine).1 CXCL14 is known to be a chemoattractant for monocyte and dendritic cells.2 CXCL14 might play a pivotal role in the pathobiology of pancreatic cancer, probably by regulating cancer invasion.3
NCBI Gene Bank ID
References & Citations
1. Moser, B. et al. (2004) Cytokine 44: 248
2. Chihara, K. et al. (2007) Biochem Biophys Res Commun. 364: 1037
3. Friess, H. et al. (2008) Cancer Lett. 259: 209
Products are for research use only. Not for use in diagnostic or therapeutic procedures.