CXC chemokine ligand 16 (CXCL16), also known as SR-PSOX, is a type I transmembrane protein. It acts as a scavenger receptor on macrophages, which specifically binds to oxidized low density lipoprotein, suggesting that it may be involved in pathophysiology such as atherogenesis (1). CXCL16 interacts with the chemokine receptor CXCR6, also known as Bonzo. It is found in liver tissue and influences the uptake, subcellular localization and cytokine profile induced by D oligonucleotides (2-3). Dendritic cells found in the T cell zones of lymphoid organs and cells found in the red pulp of the spleen produce CXCL16 (4). Cells that bind and migrate in response to CXCL16 include several subsets of T cells and natural killer T (NKT) cells (4). Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha. It may play a pro-inflammatory role in Inflammatory Bowel Disease (IBD), particularly Crohn's disease (5).
>97% by SDS-PAGE and analyzed by silver stain.
<1.0 EU/µg as determined by the LAL method
The biological activity of Human CXCL16 was determined by its ability to induce chemotaxis of mouse CXCR6 transfected BaF/3 cells. The expected ED50 is typically 2.5 - 12 ng/ml.
Protein Accession No.
Amino Acid Sequence
negsvtgscy cgkrissdsp psvqfmnrlr khlrayhrcl yytrfqllsw svcggnkdpw vqelmscldl kecghaysgi vahqkhllp
N-terminal Sequence Analysis
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human CXCL16 is Mr 10.2 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 10 kDa.
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
Next Day Ambient
NCBI Gene Bank
References & Citations
1. Mummidi, S. et al. (2004) J. Biol. Chem. 279:3188
2. Adams, DH. et al. (2005) J. Immunol. 174:1055
3. Clinman, DM. et al. (2006) J. Immunol. 177:1575
4. Matloubian, M. et al. (2000) Nat. Immunol. 1:298
5. Broedl, UC. et al. (2008) Scand. J. Gastroenterol. 43:283
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.