Anti-Human/Primate CXCL16-Biotin

Anti-Human/Primate CXCL16-Biotin

Product No.: C849

[product_table name="All Top" skus="C849"]

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Target
CXCL16
Product Type
Polyclonal Antibody
Alternate Names
Chemokine (C-X-C Motif) Ligand 16, SCYB16, SR-PSOX, CXCLG16, Zmynd15, AV290116, BB024863, 0910001K24Rik
Applications
ELISA Det
,
FC
,
IHC FFPE
,
WB

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Goat
Immunogen
E. coli-Derived Recombinant Human CXCL16 (Accession # NP_071342)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
This lyophilized biotinylated polyclonal antibody should be stored desiccated at -20°C. The reconstituted conjugate is stable for at least one month when stored at 2 - 8°C. For long term storage aseptically aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid More Than One Freeze Thaw Cycle.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CXCL16. A suggested method would be to stain cells expressing CXCL16 with 3-10 µg per 1 x 106 cells in a total staining volume of 100 µl followed by PN:A104.
Western Blotting: To detect Human CXCL16 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human CXCL16 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
ELISA Detection: Suitable for use at concentration of 0.1-0.4 µg/mL. Use in combination with: Capture Reagent: Human/Primate CXCL16 Antibody. Standard: Recombinant Human CXCL16 (Histidine-tagged) Protein.
Additional Applications Reported In Literature ?
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is PN:A106. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Human CXCL16 recognizes Human CXCL16. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Chemokine (C-X-C motif) ligand 16 (CXCL16), also known as SR-PSOX, is a transmembrane protein that facilitates uptake of low density lipoproteins by macrophages, resulting in foam cell formation.1 CXCL16 is found in liver tissue2 and influences the uptake, subcellular localization, and cytokine profile induced by D oligonucleotides.3 CXCL16 may play a pro-inflammatory role in Inflammatory Bowel Disease (IBD), particularly Crohn's disease.4

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Mummidi, S. et al. (2004) J Biol Chem. 279: 3188
2. Adams, DH. et al. (2005) J Immunol. 174: 1055
3. Clinman, DM. et al. (2006) J Immunol. 177: 1575
4. Broedl, UC. et al. (2008) Scand J Gastroenterol. 43: 283
ELISA Det
Flow Cytometry
IHC FFPE
General Western Blot Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.