Purified Recombinant Mouse CXCL16 (>98%)
<0.1 EU/µg as determined by the LAL method
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 1 µg/ml. A suitable detection antibody is PN:C1505 at a concentration of approximately 25 ng/ml. A suggested standard for this assay is PN:C847.
Western Blotting: To detect Mouse CXCL16 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse CXCL16 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:C847.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Mouse CXCL16 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse CXCL16 (at 7.5 ng/ml) is 0.15 - 0.6 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Anti-Mouse CXCL16 (Clone 142417) recognizes an epitope on Mouse CXCL16. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody showed no cross-reactivity in direct ELISAs or western blots with the chemokines tested.
Chemokine (C-X-C motif) ligand 16 (CXCL16), also known as SR-PSOX, is a transmembrane protein that facilitates uptake of low density lipoproteins by macrophages, resulting in foam cell formation.1 CXCL16 is found in liver tissue2 and influences the uptake, subcellular localization, and cytokine profile induced by D oligonucleotides.3 CXCL16 may play a pro-inflammatory role in Inflammatory Bowel Disease (IBD), particularly Crohn's disease.4
References & Citations
1.) Mummidi, S. et al. (2004) J Biol Chem. 279: 3188
2.) Adams, DH. et al. (2005) J Immunol. 174: 1055
3.) Clinman, DM. et al. (2006) J Immunol. 177: 1575
4.) Broedl, UC. et al. (2008) Scand J Gastroenterol. 43: 283
5.) Christian L. et al. (2021) Cell Reports 35:6, 109118 Journal Link
6.) Christian, L. et al. (2021) Cell Reports 35(6): 109118 Journal Link
Products are for research use only. Not for use in diagnostic or therapeutic procedures.