Anti-Mouse CXCL16 (Clone 142417)

Pricing & Details

Product No.C1430
Clone
142417
Protein
CXCL16
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
SCYB16, SR-PSOX, CXCLG16, Zmynd15, AV290116, BB024863, 0910001K24Rik
Isotype
Rat
IgG2a
Applications
ELISA Cap
,
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C1430-25 µg
25 µg
$195.00
In stock
Max:
Min: 1
Step: 1
C1430-100 µg
100 µg
$445.00
In stock
Max:
Min: 1
Step: 1
C1430-500 µg
500 µg
$995.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Purified Recombinant Mouse CXCL16 (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 1 µg/ml. A suitable detection antibody is PN:C1505 at a concentration of approximately 25 ng/ml. A suggested standard for this assay is PN:C847.
Western Blotting: To detect Mouse CXCL16 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse CXCL16 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:C847.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Mouse CXCL16 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse CXCL16 (at 7.5 ng/ml) is 0.15 - 0.6 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Anti-Mouse CXCL16 (Clone 142417) recognizes an epitope on Mouse CXCL16. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody showed no cross-reactivity in direct ELISAs or western blots with the chemokines tested.
Background
Chemokine (C-X-C motif) ligand 16 (CXCL16), also known as SR-PSOX, is a transmembrane protein that facilitates uptake of low density lipoproteins by macrophages, resulting in foam cell formation.1 CXCL16 is found in liver tissue2 and influences the uptake, subcellular localization, and cytokine profile induced by D oligonucleotides.3 CXCL16 may play a pro-inflammatory role in Inflammatory Bowel Disease (IBD), particularly Crohn's disease.4

Antigen Details

Protein
PubMed

References & Citations

1.) Mummidi, S. et al. (2004) J Biol Chem. 279: 3188
2.) Adams, DH. et al. (2005) J Immunol. 174: 1055
3.) Clinman, DM. et al. (2006) J Immunol. 177: 1575
4.) Broedl, UC. et al. (2008) Scand J Gastroenterol. 43: 283
5.) Christian L. et al. (2021) Cell Reports 35:6, 109118 Journal Link
6.) Christian, L. et al. (2021) Cell Reports 35(6): 109118 Journal Link
Elisa Sandwich Protocol
General Western Blot Protocol

Formats Available

Prod No.
Description
C1430
Products are for research use only. Not for use in diagnostic or therapeutic procedures.