Anti-Human CXCL16

Anti-Human CXCL16

Product No.: C848

[product_table name="All Top" skus="C848"]

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Target
CXCL16
Product Type
Polyclonal Antibody
Alternate Names
Chemokine (C-X-C Motif) Ligand 16, SCYB16, SR-PSOX, CXCLG16, Zmynd15, AV290116, BB024863, 0910001K24Rik
Applications
CyTOF®
,
ELISA Cap
,
FC
,
IHC FFPE
,
N
,
WB

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Product Size
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Goat
Immunogen
E. coli-Derived Recombinant Human CXCL16
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CXCL16. A suggested method would be to stain cells expressing CXCL16 with 3-10 µg per 1 x 106 cells in a total staining volume of ≤100 µl followed by PN:G641.
Western Blotting: To detect Human CXCL16 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human CXCL16 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
ELISA Capture: Suitable for use at concentration of 0.2-0.8 µg/mL. Use in combination with: Detection Reagent: Human/Primate CXCL16 Biotinylated Antibody. Standard: Recombinant Human CXCL16 (Histidine-tagged) Protein.
Additional Applications Reported In Literature ?
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 3-10 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Neutralization: This antibody is useful for neutralization of Human CXCL16 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human CXCL16 (at 20 ng/ml) is 0.2-0.7 µg/ml.
CyTOF-ready:Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Human Chemokine (C-X-C Motif) Ligand 16 (CXCL16) recognizes Human CXCL16. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Chemokine (C-X-C motif) ligand 16 (CXCL16), also known as SR-PSOX, is a transmembrane protein that facilitates uptake of low density lipoproteins by macrophages, resulting in foam cell formation.1 CXCL16 is found in liver tissue2 and influences the uptake, subcellular localization, and cytokine profile induced by D oligonucleotides.3 CXCL16 may play a pro-inflammatory role in Inflammatory Bowel Disease (IBD), particularly Crohn's disease.4

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Mummidi, S. et al. (2004) J Biol Chem. 279: 3188
2. Adams, DH. et al. (2005) J Immunol. 174: 1055
3. Clinman, DM. et al. (2006) J Immunol. 177: 1575
4. Broedl, UC. et al. (2008) Scand J Gastroenterol. 43: 283
CyTOF®
Elisa Sandwich Protocol
Flow Cytometry
IHC FFPE
N
General Western Blot Protocol
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.