Recombinant Human GM-CSF

Pricing & Details

Product. No.G124
Alternate Names
Granulocyte Macrophage Colony Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-Alpha
Expression Host
E. coli
Reactivity Species
Human
Prod No.
Size
Price
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Qty
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G124-10 µg
10 µg
$219.00
In stock
Max:
Min: 1
Step: 1
G124-50 µg
50 µg
$799.00
In stock
Max:
Min: 1
Step: 1
G124-500 µg
500 µg
$1,890.00
In stock
Max:
Min: 1
Step: 1

Data

Background

Granulocyte-Macrophage Colony Stimulating Factor is a 22 kD, pleiotropic cytokine that is a white blood cell growth factor. It controls the production and function of blood cells by stimulating stem cells to produce granulocytes and monocytes. GM-CSF differs from G-CSF in that it affects more cell types including macrophages and eosinophils. Moreover, GM-CSF is part of the immune/inflammatory cascade, a process crucial for fighting infection. Interestingly, GM-CSF expression may have pathological implications. Autocrine expression of GM-CSF in myeloid leukemia cells is suspected to play a role in neoplasia, the formation of a new and abnormal growth of tissue. Additionally, GM-CSF expression has also been documented in certain solid tumors. There have also been reports of GM-CSF in synovial fluid from patients with arthritis suggesting that GM-CSF may play a role in tissue damage associated with the inflammatory process. Blocking GM-CSF is thought to have therapeutic potential by reducing inflammation. Some drugs are currently being developed to block GM-CSF.

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human GM-CSF was determined by a cell proliferation assay using the factor-dependent cell line, TF-1 (Kitamura, T. et al., 1989, J. Cell Physiol. 140:323 - 343). The expected ED50 for this effect is typically 6 - 30 pg/ml.
Expression Host
E. coli
Protein Accession No.
CAA26822
Amino Acid Sequence
apa rspspstqpw ehvnaiqear rllnlsrdta aemnetvevi semfdlqept clqtrlelyk qglrgsltkl kgpltmmash ykqhcpptpe tscatqiitf esfkenlkdf llvipfdcwe pvqe
N-terminal Sequence Analysis
Ala18
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human GM-CSF is Mr 14 kDa. Additionally, the actual molecular weight as observed by migration on SDS-PAGE is 14 kDa (reducing conditions).
Formulation
This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.
Storage and Stability
This protein is stable for up to twelve months when stored at -20°C to -70°C. After thawing and addition of a carrier protein, this protein may be stored at 2°C to 8°C for one month or for long-term storage, aliquot and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Parker, MW. et al. (2008) Cell 134:496
2. Esnault, S. et al. (2002) Arch. Immunol. Ther. Exp. (Warsz.) 50:121
3. Yong, KL. et al. (1993) J. Immunol. 150:2449
4. Whitsett, JA. et al. (2002) Annu. Rev. Physiol. 64:775
5. Armitage, JO. et al. (1992) Sem. Hematol. 29:14
6. Eksioglu, EA. et al. (2007) Exp. Hematol. 35:1163
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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