Anti-Rat GM-CSF (Clone 83329)

Pricing & Details

Product No.G672
Clone
83329
Protein
GM-CSF
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Granulocyte Macrophage Colony Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-Alpha
Isotype
Mouse
IgG2a
Applications
ELISA Cap
,
N
Prod No.
Size
Price
Avail.
Qty
Add to cart
G672-25 µg
25 µg
$190.00
In stock
Max:
Min: 1
Step: 1
G672-500 µg
500 µg
$525.00
In stock
Max:
Min: 1
Step: 1
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Antibody Details

Product Details

Reactivity Species
Rat
Host Species
Mouse
Immunogen
Purified Recombinant Rat GM-CSF (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:G691 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:G596.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Rat GM-CSF bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Rat GM-CSF (at 0.5 ng/ml) is 0.2 - 0.8 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 83329 recognizes an epitope on Rat GM-CSF. This antibody shows no cross-reactivity with rhGM-CSF, rmGM-CSF, or rpGM-CSF.
Background
Granulocyte-Macrophage Colony Stimulating Factor is a 22 kD, pleiotropic cytokine that is a white blood cell growth factor. It controls the production and function of blood cells by stimulating stem cells to produce granulocytes and monocytes. GM-CSF differs from G-CSF in that it affects more cell types including macrophages and eosinophils. Moreover, GM-CSF is part of the immune/inflammatory cascade, a process crucial for fighting infection. Interestingly, GM-CSF expression may have pathological implications. Autocrine expression of GM-CSF in myeloid leukemia cells is suspected to play a role in neoplasia, the formation of a new and abnormal growth of tissue. Additionally, GM-CSF expression has also been documented in certain solid tumors. There have also been reports of GM-CSF in synovial fluid from patients with arthritis suggesting that GM-CSF may play a role in tissue damage associated with the inflammatory process. Blocking GM-CSF is thought to have therapeutic potential by reducing inflammation. Some drugs are currently being developed to block GM-CSF.

Antigen Details

Protein
PubMed

References & Citations

1. Parker, MW. et al. (2008) Cell 134: 496
2. Whitsett, JA. et al. (2002) Annual Review of Physiology 64: 775
Elisa Sandwich Protocol

Formats Available

Prod No.
Description
G672
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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