Recombinant Rat GM-CSF

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Pricing & Details

Product. No.G596
Alternate Names
Granulocyte Macrophage Colony Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-Alpha
Expression Host
E. coli
Species
Rat
Prod No.
Size
Price
Avail.
Qty
Add to cart
G596-5 µg
5 µg
$139.00
In stock
Max:
Min: 1
Step: 1
G596-25 µg
25 µg
$349.00
In stock
Max:
Min: 1
Step: 1

Background

Granulocyte-Macrophage Colony Stimulating Factor is a 22 kD, pleiotropic cytokine that is a white blood cell growth factor. It controls the production and function of blood cells by stimulating stem cells to produce granulocytes and monocytes. GM-CSF differs from G-CSF in that it affects more cell types including macrophages and eosinophils. Moreover, GM-CSF is part of the immune/inflammatory cascade, a process crucial for fighting infection. Interestingly, GM-CSF expression may have pathological implications. Autocrine expression of GM-CSF in myeloid leukemia cells is suspected to play a role in neoplasia, the formation of a new and abnormal growth of tissue. Additionally, GM-CSF expression has also been documented in certain solid tumors. There have also been reports of GM-CSF in synovial fluid from patients with arthritis suggesting that GM-CSF may play a role in tissue damage associated with the inflammatory process. Blocking GM-CSF is thought to have therapeutic potential by reducing inflammation. Some drugs are currently being developed to block GM-CSF.

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Expression Host
E. coli
Protein Accession No.
Amino Acid Sequence
aptrspnpvt rpwkhvdaik ealsllndmr aleneknedv diisnefsiq rptcvqtrlk lykqglrgnl tklngaltmi ashyqtncpp tpetdceiev ttfedfiknl kgflfdipfd cwkpvqk
N-terminal Sequence Analysis
Met & Ala1
State of Matter
Solution
Predicted Molecular Mass
The predicted molecular weight of Recombinant Rat GM-CSF is Mr 14.7 kDa.
Formulation
This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.
Storage and Stability
Working aliquots of this recombinant protein solution are stable for up to twelve months at -20°C to -70°C in a manual defrost freezer. Upon thawing, in the presence of a carrier protein, this recombinant protein can be stored at 2° - 8°C for one month without detectable loss of activity. For long-term storage, aliquot and freeze at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles.
Country of Origin
USA
Shipping
Dry Ice
PubMed
NCBI Gene Bank

References & Citations

1. Parker, MW. et al. (2008) Cell 134: 496
2. Trapnell, BC. and Whitsett, JA. (2002) Annual Review of Physiology 64: 775
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.