Anti-Human GM-CSF (Clone 3209)

Product No.: G665


Clone 3209 Target GM-CSF Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Granulocyte Macrophage Colony Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-Alpha Isotype IgG1 Applications ICC , N , WB


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Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen Purified Recombinant Human GM-CSF (Accession # P04141) Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830255 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human GM-CSF this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human GM-CSF is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:G124. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human GM-CSF bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human GM-CSF (at 0.5 ng/ml) is 0.3 - 0.5 µg/ml. Immunocytochemistry: Suitable for use at concentration of 8-25 µg/mL. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Clone 3209 recognizes an epitope on human GM-CSF. In direct ELISAs, this antibody exhibits less than 15% cross-reactivity with rmGM-CSF. Background Granulocyte-Macrophage Colony Stimulating Factor is a 22 kD, pleiotropic cytokine that is a white blood cell growth factor. It controls the production and function of blood cells by stimulating stem cells to produce granulocytes and monocytes. GM-CSF differs from G-CSF in that it affects more cell types including macrophages and eosinophils. Moreover, GM-CSF is part of the immune/inflammatory cascade, a process crucial for fighting infection. Interestingly, GM-CSF expression may have pathological implications. Autocrine expression of GM-CSF in myeloid leukemia cells is suspected to play a role in neoplasia, the formation of a new and abnormal growth of tissue. Additionally, GM-CSF expression has also been documented in certain solid tumors. There have also been reports of GM-CSF in synovial fluid from patients with arthritis suggesting that GM-CSF may play a role in tissue damage associated with the inflammatory process. Blocking GM-CSF is thought to have therapeutic potential by reducing inflammation. Some drugs are currently being developed to block GM-CSF. PubMed GM-CSF References & Citations 1. Parker, MW. et al. (2008) Cell 134: 496 2. Whitsett, JA. et al. (2002) Annual Review of Physiology 64: 775 View product citations for antibody G665 on CiteAb Technical Protocols ICC N Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human GM-CSF (Accession # P04141)

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2830255

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Human GM-CSF this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human GM-CSF is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:G124.

Additional Applications Reported In Literature ?

Neutralization: This antibody is useful for neutralization of Human GM-CSF bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human GM-CSF (at 0.5 ng/ml) is 0.3 - 0.5 µg/ml.
Immunocytochemistry: Suitable for use at concentration of 8-25 µg/mL.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone 3209 recognizes an epitope on human GM-CSF. In direct ELISAs, this antibody exhibits less than 15% cross-reactivity with rmGM-CSF.

Background

Granulocyte-Macrophage Colony Stimulating Factor is a 22 kD, pleiotropic cytokine that is a white blood cell growth factor. It controls the production and function of blood cells by stimulating stem cells to produce granulocytes and monocytes. GM-CSF differs from G-CSF in that it affects more cell types including macrophages and eosinophils. Moreover, GM-CSF is part of the immune/inflammatory cascade, a process crucial for fighting infection. Interestingly, GM-CSF expression may have pathological implications. Autocrine expression of GM-CSF in myeloid leukemia cells is suspected to play a role in neoplasia, the formation of a new and abnormal growth of tissue. Additionally, GM-CSF expression has also been documented in certain solid tumors. There have also been reports of GM-CSF in synovial fluid from patients with arthritis suggesting that GM-CSF may play a role in tissue damage associated with the inflammatory process. Blocking GM-CSF is thought to have therapeutic potential by reducing inflammation. Some drugs are currently being developed to block GM-CSF.

PubMed

GM-CSF

References & Citations

1. Parker, MW. et al. (2008) Cell 134: 496
2. Whitsett, JA. et al. (2002) Annual Review of Physiology 64: 775

View product citations for antibody G665 on CiteAb

ICC

Certificate of Analysis

Request COA

Prod No.	Description
G665	Anti-Human GM-CSF (Clone 3209)
G675	Anti-Human GM-CSF (Clone 6804)
G596	Recombinant Rat GM-CSF
G128	Recombinant Mouse GM-CSF
G672	Anti-Rat GM-CSF (Clone 83329)
G681	Anti-Rat GM-CSF
G119	Anti-Mouse GM-CSF
G124	Recombinant Human GM-CSF
G142	Anti-Human GM-CSF – Biotin
G691	Anti-Rat GM-CSF – Biotin
G143	Anti-Mouse GM-CSF – Biotin

Formats Available

Prod No.	Description
G665	Anti-Human GM-CSF (Clone 3209)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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Anti-Human GM-CSF (Clone 3209)