Anti-Human GM-CSF (Clone 3209)
Anti-Human GM-CSF (Clone 3209)
Product No.: G665
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Clone 3209 Target GM-CSF Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Granulocyte Macrophage Colony Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-Alpha Isotype IgG1 Applications ICC , N , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified Recombinant Human GM-CSF (Accession # P04141) Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830255 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human GM-CSF this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human GM-CSF is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:G124. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human GM-CSF bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human GM-CSF (at 0.5 ng/ml) is 0.3 - 0.5 µg/ml. Immunocytochemistry: Suitable for use at concentration of 8-25 µg/mL. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Clone 3209 recognizes an epitope on human GM-CSF. In direct ELISAs, this antibody exhibits less than 15% cross-reactivity with rmGM-CSF. Background Granulocyte-Macrophage Colony Stimulating Factor is a 22 kD, pleiotropic cytokine that is a white blood cell growth factor. It controls the production and function of blood cells by stimulating stem cells to produce granulocytes and monocytes. GM-CSF differs from G-CSF in that it affects more cell types including macrophages and eosinophils. Moreover, GM-CSF is part of the immune/inflammatory cascade, a process crucial for fighting infection. Interestingly, GM-CSF expression may have pathological implications. Autocrine expression of GM-CSF in myeloid leukemia cells is suspected to play a role in neoplasia, the formation of a new and abnormal growth of tissue. Additionally, GM-CSF expression has also been documented in certain solid tumors. There have also been reports of GM-CSF in synovial fluid from patients with arthritis suggesting that GM-CSF may play a role in tissue damage associated with the inflammatory process. Blocking GM-CSF is thought to have therapeutic potential by reducing inflammation. Some drugs are currently being developed to block GM-CSF. Antigen DetailsPubMed References & Citations1. Parker, MW. et al. (2008) Cell 134: 496 2. Whitsett, JA. et al. (2002) Annual Review of Physiology 64: 775 Technical ProtocolsICC N  |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
