Recombinant Human HER2 – Purified

HER2 (Human Epidermal Growth Factor Receptor 2), also known as ErbB2, CD340, or proto-oncogene Neu, is a 185 kDa transmembrane glycoprotein member of the epidermal growth factor receptor (EGFR) family. Unlike other ErbB family members, the HER2 protein does not have a direct ligand-binding domain but instead acts as a preferred heterodimerization partner for other ligand-bound receptors (HER1, HER3, and HER4).

This recombinant human HER2 protein is essential for researchers studying cell proliferation, survival, and differentiation pathways, particularly the MAPK and PI3K/Akt signaling cascades. Overexpression or amplification of the ERBB2 gene is a well-established biomarker in approximately 20-30% of breast cancers and is also prevalent in gastric (stomach) and ovarian carcinomas.

Our high-purity, biologically active recombinant HER2 is designed for use in a variety of applications, including ELISA, functional assays, surface plasmon resonance (SPR), and as an immunogen for anti-HER2 antibody development.

Human

TQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGACQPCPINCTHSCVDLDDKGCPAEQRASPLT

Lyophilized

70 kDa

Reconstitute at 1mg/ml using filtered deionized water. Gently mix by vortexing and/or inversion for 5 minutes or until fully dissolved. Centrifuge if necessary.

This lyophilized protein is stable for twelve months when stored at -20°C to -70°C. After aseptic reconstitution, this protein may be stored for one month at 2°C to 8°C or for three months at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Ambient

HER2 (ErbB2) forms dimers with EGFR, ErbB2, ErbB3, and ErbB4

Human

Research Use Only

1 https://www.ncbi.nlm.nih.gov/gene/2064
2 Dean L, Kane M. Pertuzumab Therapy and ERBB2 Genotype. 2015 Sep 10 [Updated 2021 Jan 21]. In: Pratt VM, Scott SA, Pirmohamed M, et al., editors. Medical Genetics Summaries [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2012-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK315949/
3 Moasser MM. Oncogene. 26(45):6469-6487. 2007.
4 Metzger-Filho O, Winer EP, Krop I. Clin Cancer Res. 19(20):5552-5556. 2013.
5 Galogre M, Rodin D, Pyatnitskiy M, et al. Crit Rev Oncol Hematol. 186:103997. 2023.
6 Oh DY, Bang YJ. Nat Rev Clin Oncol. 17(1):33-48. 2020.