Insulin-like growth factor binding protein 2, also known as IGFBP2, is an extracellular unglycosylated polypeptide that specifically binds IGF and modulates its effects.1 IGFBP-2 has direct effects on breast cancer proliferation via stimulation of critical signaling pathways.2
>95% by SDS-PAGE and analyzed by silver stain.
<1.0 EU/µg as determined by the LAL method
The biological activity of Human IGFBP-2 was determined by its ability to inhibit the biological activity of rhIGF-II on MCF-7 cells (Karey, K.P. et al., 1988, Cancer Research 48:4083). The expected ED50 for this effect is 0.03 - 0.09 μg/ml in the presence of 14 ng/ml rhIGF-II.
Protein Accession No.
Amino Acid Sequence
mevlfrcppc tperlaacgp ppvappaava avaggarmpc aelvrepgcg ccsvcarleg eacgvytprc gqglrcyphp gselplqalv mgegtcekrr daeygaspeq vadngddhse gglvenhvds tmnmlggggs agrkplksgm kelavfrekv teqhrqmgkg gkhhlgleep kklrpppart pcqqeldqvl eristmrlpd ergplehlys lhipncdkhg lynlkqckms lngqrgecwc vnpntgkliq gaptirgdpe chlfyneqqe acgvhtqrmq
N-terminal Sequence Analysis
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human IGFBP-2 is Mr 31 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 36 kDa.
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
Next Day Ambient
NCBI Gene Bank
References & Citations
1. Luthman, H. et al. (1991) Biochem Biophys Res Commun. 176: 1250
2. Disis, ML. et al. (2008) Cancer Res. 68: 8400
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.