NS0-Derived Recombinant Mouse IGFBP-2
<0.1 EU/µg as determined by the LAL method
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse IGFBP-2 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse IGFBP-2 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Mouse IGFBP-2 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse IGFBP-2 (at 0.75 ng/ml) is 5 - 20 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Rat Anti-Mouse Insulin-Like Growth Factor Binding Protein 2 (IGFBP-2) (Clone 150101) recognizes an epitope on Mouse IGFBP-2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Insulin-like growth factor binding protein 2, also known as IGFBP2, is an extracellular unglycosylated polypeptide that specifically binds IGF and modulates its effects.1 IGFBP-2 has direct effects on breast cancer proliferation via stimulation of critical signaling pathways.2
NCBI Gene Bank ID
References & Citations
1. Luthman, H. et al.(1991) Biochem Biophys Res Commun. 176: 1250
2. Disis, ML. et al.(2008) Cancer Res. 68: 8400
Products are for research use only. Not for use in diagnostic or therapeutic procedures.