Recombinant Human IL-1 RAcP

Recombinant Human IL-1 RAcP

Product No.: I-578

[product_table name="All Top" skus="I-578"]

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Alternate Names
IL1RAP, IL-1R3, C3orf13, FLJ37788
Product Type
Recombinant Protein
Expression Host
HEK-293 Cells
Species
Human
Applications
ELISA Cap

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Product Size
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Background

IL-1R accessory protein (IL-1RAcP) is a transmembrane protein that interacts with IL-1R and is required for IL-1 signal transduction. IL-1RAcP is an indispensible molecule in the IL-1 receptor signal transduction complex, necessary to link events on the plasma membrane level to downstream signaling pathways, allowing IL-1-dependent activation of transcription factors and gene expression.1 IL-1R AcP represents a novel target for blocking IL-1 function in human disease.2

Protein Details

Purity
>90% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Fusion Protein Tag
HEK293-derived human IL-1 RAcP/IL-1 R3 protein Ser21-Glu359, with a C-terminal 6-His tag
Protein Accession No.
Amino Acid Sequence
sercddwgld tmrqiqvfed eparikcplf ehflkfnyst ahsagltliw ywtrqdrdle epinfrlpen riskekdvlw frptllndtg nytcmlrntt ycskvafple vvqkdscfns pmklpvhkly ieygiqritc pnvdgyfpss vkptitwymg cykiqnfnnv ipegmnlsfl ialisnngny tcvvtypeng rtfhltrtlt vkvvgspkna vppvihspnd hvvyekepge ellipctvyf sflmdsrnev wwtidgkkpd ditidvtine sishsrtede trtqilsikk vtsedlkrsy vcharsakge vakaakvkqk vpaprytvei egrmdpkscd kthtcppcpa pellggpsvf lfppkpkdtl misrtpevtc vvvdvshedp evkfnwyvdg vevhnaktkp reeqynstyr vvsvltvlhq dwlngkeykc kvsnkalpap iektiskakg qprepqvytl ppsrdeltkn qvsltclvkg fypsdiavew esngqpenny kttppvldsd gsfflysklt vdksrwhhhh hh qqgnvfscsv mhealhnhyt qkslslspgk
N-terminal Sequence Analysis
Ser21
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human IL-1 RAcP is Mr 40 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 55-65kDa.
Predicted Molecular Mass
40
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Martin, MU. et al. (1997) J Biol Chem. 272: 7727
2. Labow, MA. et al. (1998) J Immunol. 161: 5614
Elisa Sandwich Protocol
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.