Recombinant Human MCP-1

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Pricing & Details

Product. No.M156
Alternate Names
CCL2, GDCF-2, GDCF-2 HC11, HC11, HSMCR30, MCAF, MGC9434, SCYA2, SMC-CF, JE
Expression Host
E. coli
Species
Human
Prod No.
Size
Price
Avail.
Qty
Add to cart
M156-10 µg
10 µg
$219.00
In stock
Max:
Min: 1
Step: 1
M156-50 µg
50 µg
$449.00
In stock
Max:
Min: 1
Step: 1

Data

Background

Monocyte chemotactic protein-1 (MCP-1) is an essential chemokine produced predominantly by macrophages and endothelial cells and is a potent chemotactic factor for monocytes.1,2,3 It can induce the proliferation and activation of killer cells known as CHAK (CC-Chemokine-activated killer). MCP-1 is also a potent activator of human basophils, induces degranulation and the release of histamines. MCP-1 is involved in the pathogenesis of atherosclerosis and myocardial infarction.4

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human MCP-1 was determined by its monocyte chemotactic activity and its ability to chemoattract hCCR2A transfected mouse BaF/3 cells. The expected ED50 for these effects are typically 5 - 20 ng/ml and 10 - 60 ng/ml, respectively.
Expression Host
E. coli
Protein Accession No.
Amino Acid Sequence
qpdaina pvtccynftn rkisvqrlas yrritsskcp keavifktiv akeicadpkq kwvqdsmdhl dkqtqtpkt
N-terminal Sequence Analysis
Gln24
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human MCP-1 is Mr 8.7 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is 10 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Yoshimura, T. et al. (1989) J. Exp. Med. 169: 1449
2. Oppenheim, JJ. et al. (1989) J. Exp. Med. 169: 1485
3. Rollins, BJ. et al. (1997) Blood 90: 909
4. Benjamin, EJ. et al. (2005) Ciculation 112: 1113
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.