Anti-Human MCP-1 (Clone 24822)

Pricing & Details

Product No.M152
Clone
24822
Protein
MCP-1
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CCL2, GDCF-2, GDCF-2 HC11, HC11, HSMCR30, MCAF, MGC9434, SCYA2, SMC-CF, JE
Isotype
Mouse
IgG1
Applications
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
M152-25 µg
25 µg
$205.00
In stock
Max:
Min: 1
Step: 1
M152-100 µg
100 µg
$389.00
In stock
Max:
Min: 1
Step: 1
M152-500 µg
500 µg
$920.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human MCP-1 (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human MCP-1 this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human MCP-1 is 5 and 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M156.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human MCP-1 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human MCP-1 (at 0.15 ng/ml) is 0.9 - 4.5 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Monocyte Chemotactic Protein-1 (MCP-1) recognizes Human MCP-1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
MCP-1 is an essential chemokine produced predominantly by macrophages and endothelial cells and is a potent chemotactic factor for monocytes.1,2,3 It can induce the proliferation and activation of killer cells known as CHAK (CC-Chemokine-activated killer). MCP-1 is also a potent activator of human basophils, induces degranulation and the release of histamines. MCP-1 is involved in the pathogenesis of atherosclerosis and myocardial infarction.4

Antigen Details

Protein
PubMed

References & Citations

1. Yoshimura, T. et al. (1989) J. Exp. Med. 169: 1449
2. Oppenheim, JJ. et al. (1989) J. Exp. Med. 169: 1485
3. Rollins, BJ. et al. (1997) Blood 90: 909
4. Benjamin, EJ. et al. (2005) Ciculation 112: 1113
General Western Blot Protocol

Formats Available

Prod No.
Description
M152
Products are for research use only. Not for use in diagnostic or therapeutic procedures.