Anti-Human MCP-1 (Clone 24822)

Pricing & Details


Product No.M152 Clone 24822 Protein MCP-1 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CCL2, GDCF-2, GDCF-2 HC11, HC11, HSMCR30, MCAF, MGC9434, SCYA2, SMC-CF, JE Isotype Mouse IgG1 Applications N , WB Submit A Review	Printable Datasheet Printable SDS

Prod No.	Size	Price	Avail.	Qty	Add to cart
M152-25 µg	25 µg	225.00	In stock	Max: Min: 1 Step: 1	Add to Cart
M152-100 µg	100 µg	450.00	In stock	Max: Min: 1 Step: 1	Add to Cart
M152-500 µg	500 µg	1,025.00	In stock	Max: Min: 1 Step: 1	Add to Cart


Antibody Details Product Details Reactivity Species Human Host Species Mouse Immunogen Purified Recombinant Human MCP-1 (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831356 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human MCP-1 this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human MCP-1 is 5 and 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M156. Other Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human MCP-1 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human MCP-1 (at 0.15 ng/ml) is 0.9 - 4.5 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity Mouse Anti-Human Monocyte Chemotactic Protein-1 (MCP-1) recognizes Human MCP-1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background MCP-1 is an essential chemokine produced predominantly by macrophages and endothelial cells and is a potent chemotactic factor for monocytes.^1,2,3 It can induce the proliferation and activation of killer cells known as CHAK (CC-Chemokine-activated killer). MCP-1 is also a potent activator of human basophils, induces degranulation and the release of histamines. MCP-1 is involved in the pathogenesis of atherosclerosis and myocardial infarction.⁴ Antigen Details Protein MCP-1 PubMed MCP-1 References & Citations 1. Yoshimura, T. et al. (1989) J. Exp. Med. 169: 1449 2. Oppenheim, JJ. et al. (1989) J. Exp. Med. 169: 1485 3. Rollins, BJ. et al. (1997) Blood 90: 909 4. Benjamin, EJ. et al. (2005) Ciculation 112: 1113 Technical Protocols

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human MCP-1 (>98%)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2831356

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Human MCP-1 this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human MCP-1 is 5 and 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M156.

Other Applications Reported In Literature ?

Neutralization: This antibody is useful for neutralization of Human MCP-1 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human MCP-1 (at 0.15 ng/ml) is 0.9 - 4.5 µg/ml.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human Monocyte Chemotactic Protein-1 (MCP-1) recognizes Human MCP-1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

MCP-1 is an essential chemokine produced predominantly by macrophages and endothelial cells and is a potent chemotactic factor for monocytes.^1,2,3 It can induce the proliferation and activation of killer cells known as CHAK (CC-Chemokine-activated killer). MCP-1 is also a potent activator of human basophils, induces degranulation and the release of histamines. MCP-1 is involved in the pathogenesis of atherosclerosis and myocardial infarction.⁴

Antigen Details

Protein

MCP-1

PubMed

MCP-1

References & Citations

1. Yoshimura, T. et al. (1989) J. Exp. Med. 169: 1449
2. Oppenheim, JJ. et al. (1989) J. Exp. Med. 169: 1485
3. Rollins, BJ. et al. (1997) Blood 90: 909
4. Benjamin, EJ. et al. (2005) Ciculation 112: 1113

Prod No.	Description
M164	Recombinant Rat MCP-1
M1256	Recombinant Human MCP-1 (Mucin Stalk Chimera)
M152	Anti-Human MCP-1 (Clone 24822)
M1271	Anti-Human MCP-1 (Clone 23002)
M142	Anti-Human MCP-1
M156	Recombinant Human MCP-1
J100	Recombinant Mouse MCP-1
M1323	Rat MCP-1 ELISA Development Kit
J102	Anti-Mouse MCP-1 – Biotin
M122	Anti-Human MCP-1 – Biotin
M1109	Human MCP-1 ELISA Development Kit
J104	Mouse MCP-1 ELISA Development Kit

Formats Available

Prod No.	Description
M152	Anti-Human MCP-1 (Clone 24822)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.