Recombinant Human RANTES

RANTES also known as Chemokine (C-C motif) ligand 5 (CCL5), a CC-chemokine¹ is an important mediators of the immune and inflammatory response.² It is also a potent chemoattractant for T cells ³ and eosinophils.⁴ RANTES is involved in many inflammatory diseases, for example, bronchial asthma, delayed-type hypersensitivity reactions, viral infections, arthritis, chronic eosinophilic pneumonia, and idiopathic interstitial pneumonia.⁵

The biological activity of Human RANTES was determined by its ability to chemoattract human monocytes (Schall, T. et al., 1990, Nature 347:669 - 671) or mouse BaF/3 cells transfected with human CCR5. The expected ED₅₀ for these effects are typically 20 - 30 ng/ml and 1 - 5 ng/ml, respectively.

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Ser24

Lyophilized

The predicted molecular weight of Recombinant Human RANTES is Mr 7.8 kDa.

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Lee, SC. et al. (2004) J Neurochem. 90: 297
2. Borkowski, J. et al. (2004) Arch Immunol Ther Exp (Warsz) 52: 201
3. Geiger, H. et al. (2005) Kidney Blood Press Res 28: 48
4. Hirata, I. et al.(2009) Dig Dis Sci. 54(6):1247-52.
5. Emilie, D. et al. (1994) J Exp Med. 179: 1689