Anti-Human RANTES – (Clone 21445)

Pricing & Details

Product No.R132
Clone
21445
Protein
RANTES
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CCL5, MGC17164, SCYA5, SIS-Delta, TCP228
Isotype
Mouse
IgG1
Applications
CyTOF®
,
FC
,
IHC
,
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
R132-25 μg
25 µg
$195.00
In stock
Max:
Min: 1
Step: 1
R132-100 µg
100 µg
$389.00
In stock
Max:
Min: 1
Step: 1
R132-500 µg
500 µg
$920.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human RANTES (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing RANTES. A suggested method would be to stain cells expressing RANTES with 0.25 µg per 1-5 x 10 cells in a total staining volume of ≤200 µl followed by PN:M1189.
Western Blotting: To detect Human RANTES this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human RANTES is 50 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:R133.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human RANTES bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human RANTES is 0.01 µg/ml.
Immunohistochemistry: Suitable for use at concentration of 8-25 µg/mL.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Regulated Upon Activation Normal T Cell Expressed Sequence (RANTES) (Clone 21445) recognizes an epitope on Human RANTES. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
RANTES also known as Chemokine (C-C motif) ligand 5 (CCL5), a CC-chemokine1 is an important mediators of the immune and inflammatory response.2 It is also a potent chemoattractant for T cells 3 and eosinophils.4 RANTES is involved in many inflammatory diseases, for example, bronchial asthma, delayed-type hypersensitivity reactions, viral infections, arthritis, chronic eosinophilic pneumonia, and idiopathic interstitial pneumonia.5

Antigen Details

Protein
PubMed

References & Citations

1. Lee, SC. et al. (2004) J Neurochem. 90: 297
2. Borkowski, J. et al. (2004) Arch Immunol Ther Exp (Warsz) 52: 201
3. Geiger, H. et al. (2005) Kidney Blood Press Res 28: 48
4. Hirata, I. et al.(2009) Dig Dis Sci. 54(6):1247-52.
5. Emilie, D. et al. (1994) J Exp Med. 179: 1689
Flow Cytometry
IHC
General Western Blot Protocol

Formats Available

Prod No.
Description
R132
Products are for research use only. Not for use in diagnostic or therapeutic procedures.