Anti-Mouse RANTES (Clone 53405)

Purified Recombinant Mouse RANTES (>98%)

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

Neutralization: This antibody is useful for neutralization of Mouse CCL5 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Mouse CCL5 (at 0.05 ng/ml) is 0.1 - 0.5 µg/ml.
Immunocytochemistry: Suitable for use at concentration of 8-25 µg/mL.

Rat Anti-Mouse Regulation Upon Activation Normal T Cell Express Sequence (RANTES) (Clone 53405) recognizes an epitope on Mouse RANTES. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

RANTES also known as Chemokine (C-C motif) ligand 5 (CCL5), a CC-chemokine¹ is an important mediators of the immune and inflammatory response.² It is also a potent chemoattractant for T cells ³ and eosinophils.⁴ RANTES is involved in many inflammatory diseases, for example, bronchial asthma, delayed-type hypersensitivity reactions, viral infections, arthritis, chronic eosinophilic pneumonia, and idiopathic interstitial pneumonia.⁵

1. Lee, SC. et al. (2004) J Neurochem. 90: 297
2. Borkowski, J. et al. (2004) Arch Immunol Ther Exp (Warsz) 52: 201
3. Geiger, H. et al. (2005) Kidney Blood Press Res 28: 48
4. Hirata, I. et al.(2009) Dig Dis Sci. 54(6):1247-52.
5. Emilie, D. et al. (1994) J Exp Med. 179: 1689