BackgroundRANTES also known as Chemokine (C-C motif) ligand 5 (CCL5), a CC-chemokine1 is an important mediators of the immune and inflammatory response.2 It is also a potent chemoattractant for T cells 3 and eosinophils.4 RANTES is involved in many inflammatory diseases, for example, bronchial asthma, delayed-type hypersensitivity reactions, viral infections, arthritis, chronic eosinophilic pneumonia, and idiopathic interstitial pneumonia.5 Protein DetailsPurity >95% by SDS-PAGE and analyzed by silver stain. Endotoxin Level <0.01 EU/µg as determined by the LAL method Biological Activity The biological activity of Mouse RANTES was determined by its ability to chemoattract 2 day cultured human monocytes or mouse BaF3 cells transfected with human CCR5. The expected ED50 for these effects are typically 30 - 100 ng/ml and 7 - 35 ng/ml, respectively. Expression Host E. coli Protein Accession No. Q5XZF2 Amino Acid Sequence spygsdttpc cfaylslalp rahvkeyfyt sskcsnlavv fvtrrnrqvc anpekkwvqe yinylems
N-terminal Sequence Analysis Ser24 State of Matter Lyophilized Predicted Molecular Mass The predicted molecular weight of Recombinant Mouse RANTES is Mr 7.9 kDa. Formulation This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA) with trehalose. Storage and Stability This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. Country of Origin USA Shipping Next Day Ambient PubMed NCBI Gene Bank References & Citations1. Lee, SC. et al. (2004) J Neurochem. 90: 297 2. Borkowski, J. et al. (2004) Arch Immunol Ther Exp (Warsz) 52: 201 3. Geiger, H. et al. (2005) Kidney Blood Press Res 28: 48 4. Hirata, I. et al.(2009) Dig Dis Sci. 54(6):1247-52. 5. Emilie, D. et al. (1994) J Exp Med. 179: 1689 IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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