IFN-γ receptor ligand-binding chain (IFNγR1) or IFN-γ R α chain, also known CDw119, is member of the class II cytokine receptor family. The IFN-γ receptor is a complex of a high affinity IFN-γ-binding chain (aka, IFN-γ R α chain) and a second accessory protein required for signal transduction known as IFN-γ R β chain. Binding of IFN-γ induces receptor dimerization and internalization. Signal transduction involves Jak1 and Jak2 protein kinases and involves STAT1 activation. The IFN-γ receptor is expressed at moderate levels on virtually every cell with the exception of erythrocytes.1,2,3
>95% by SDS-PAGE and analyzed by silver stain.
<0.1 EU/µg as determined by the LAL method
The biological activity of Mouse IFN-γ R1 was determined by its ability to inhibit rmIFN-γ mediated protection of mouse L929 cells viral lysis. The expected ED50 is typically 0.4 - 2 μg/mL in the presence of 1 ng/mL of rmIFN-γ.
Protein Accession No.
Amino Acid Sequence
altst edpeppsvpv ptnvliksyn lnpvvcweyq nmsqtpiftv qvkvysgswt dsctnisdhc cniyeqimyp dvsawarvka kvgqkesdya rskeflmclk gkvgppglei rrkkeeqlsv lvfhpevvvn gesqgtmfgd gstcytfdyt vyvehnrsge ilhtkhtvek eecnetlcel nisvstldsr ycisvdgiss fwqvrteksk dvcippfhdd rkdiegrmdp kscdkthtcp pcpapellgg psvflfppkp kdtlmisrtp evtcvvvdvs hedpevkfnw yvdgvevhna ktkpreeqyn styrvvsvlt vlhqdwlngk eykckvsnka lpapiektis kakgqprepq vytlppsrde ltknqvsltc lvkgfypsdi avewesngqp ennykttppv ldsdgsffly skltvdksrw qqgnvfscsv mhealhnhyt qkslslspgk
N-terminal Sequence Analysis
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse IFN-γ R1 is Mr 52.4 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 70-90 kDa.
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
Next Day Ambient
References & Citations
1. Calderon, J. et al. (1988) PNAS 85: 4837
2. Garcia-Sastre, A. et al. (2002) Int. Rev. Immunol. 21: 305
3. Fish, E. et al. (2002) J. Interferon Cytokine Res. 22: 835
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.