Anti-Human IFN-γ R1
- -
- -
Antibody DetailsProduct DetailsReactive Species Human Host Species Goat Immunogen NS0-Derived Recombinant Human Interferon-γ Receptor 1 Extracellular Domain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered in modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 with concentration 1.648 and no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830851 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human IFN-γ R1 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human IFN-γ R1 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human IFN-γ R1 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IFN-γ R1 (at 2 ng/ml) is 0.5 - 2.0 µg/ml. Immunocytochemistry: Suitable for use at concentration of 5-15 µg/mL. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Anti-Human Interferon Gamma Receptor 1 (IFN-γ R1) recognizes Human IFN-γ R1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background IFN-γ receptor ligand-binding chain (IFNγR1) or IFN-γ R α chain, also known CDw119, is member of the class II cytokine receptor family. The IFN-γ receptor is a complex of a high affinity IFN-γ-binding chain (aka, IFN-γ R α chain) and a second accessory protein required for signal transduction known as IFN-γ R β chain. Binding of IFN-γ induces receptor dimerization and internalization. Signal transduction involves Jak1 and Jak2 protein kinases and involves STAT1 activation. The IFN-γ receptor is expressed at moderate levels on virtually every cell with the exception of erythrocytes.1,2,3 Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org References & Citations1. Calderon, J. et al. (1988) PNAS 85: 4837 2. Garcia-Sastre, A. et al. (2002) Int. Rev. Immunol. 21: 305 3. Fish, E. et al. (2002) J. Interferon Cytokine Res. 22: 835 Technical ProtocolsICC N  |
Related Products
- -
- -
Prod No. | Description |
|---|---|
I-730 | |
I-691 | |
I-538 | |
I-577 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
