Anti-Human IFN-γ Rα Chain (CD119)

Product No.: I-691


Clone 92101 Target IFNγ Rα Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Interferon Gamma Receptor 1, CD119, IFN-γR, IFN-γRα, IFN-α R1, CDw119 Isotype IgG1 Applications ELISA Indirect , FC , N , WB


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Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen NS0-Derived Recombinant Human IFN-γ Receptor 1 (Accession # P15260.1) Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830812 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IFN-γ R1. A suggested method would be to stain cells expressing IFN-γ R1 with 0.25 µg per 1 - 2.5 x 10⁶ cells in a total staining volume of ≤200 µl followed by PN:M1189. Western Blotting: To detect Human IFN-γ R1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human IFN-γ R1 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human IFN-γ R1 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human IFN-γ R1 (at 2 ng/ml) is 0.01 - 0.03 µg/ml. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Mouse Anti-Human Interferon Gamma Receptor 1 (IFN-γ R1) (Clone 92101) recognizes an epitope on Human IFN-γ R1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background IFNγ Rα, or IFNγ R1, is a 90-100 kD type I transmembrane protein that is structurally related to IL-10 receptor. IFNγ Receptor consists of α and ß chains and requires association of JAK1, JAK2 and Stat1 for IFN-γ signal transduction which induces tyrosine phosphorylation of IFN-γ Rα leading to the formation of a docking site on the activated receptor for Stat1, which specifically activates IFN-γ induced gene transcription. Antigen Distribution The IFN-γ receptor is expressed at moderate levels on virtually every cell with the exception of erythrocytes. PubMed IFNγ Rα NCBI Gene Bank ID 3459 UniProt.org Information on Uniprot.org References & Citations 1. Calderon, J. et al. (1988) PNAS 85: 4837 2. Garcia-Sastre, A. et al. (2002) Int. Rev. Immunol. 21: 305 3. Fish, E. et al. (2002) J. Interferon Cytokine Res. 22: 835 View product citations for antibody I-691 on CiteAb Technical Protocols N Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

NS0-Derived Recombinant Human IFN-γ Receptor 1 (Accession # P15260.1)

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2830812

Applications and Recommended Usage?
Quality Tested by Leinco

Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IFN-γ R1. A suggested method would be to stain cells expressing IFN-γ R1 with 0.25 µg per 1 - 2.5 x 10⁶ cells in a total staining volume of ≤200 µl followed by PN:M1189.
Western Blotting: To detect Human IFN-γ R1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human IFN-γ R1 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Additional Applications Reported In Literature ?

Neutralization: This antibody is useful for neutralization of Human IFN-γ R1 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human IFN-γ R1 (at 2 ng/ml) is 0.01 - 0.03 µg/ml.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human Interferon Gamma Receptor 1 (IFN-γ R1) (Clone 92101) recognizes an epitope on Human IFN-γ R1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

IFNγ Rα, or IFNγ R1, is a 90-100 kD type I transmembrane protein that is structurally related to IL-10 receptor. IFNγ Receptor consists of α and ß chains and requires association of JAK1, JAK2 and Stat1 for IFN-γ signal transduction which induces tyrosine phosphorylation of IFN-γ Rα leading to the formation of a docking site on the activated receptor for Stat1, which specifically activates IFN-γ induced gene transcription.

Antigen Distribution

The IFN-γ receptor is expressed at moderate levels on virtually every cell with the exception of erythrocytes.

PubMed

IFNγ Rα

NCBI Gene Bank ID

3459

UniProt.org

Information on Uniprot.org

References & Citations

1. Calderon, J. et al. (1988) PNAS 85: 4837
2. Garcia-Sastre, A. et al. (2002) Int. Rev. Immunol. 21: 305
3. Fish, E. et al. (2002) J. Interferon Cytokine Res. 22: 835

View product citations for antibody I-691 on CiteAb

Certificate of Analysis

Request COA

Prod No.	Description
I-691	Anti-Human IFN-γ Rα Chain (CD119)
I-538	Recombinant Mouse IFN-γ R1
I-577	Recombinant Human IFN-γ R1

Formats Available

Prod No.	Description
I-691	Anti-Human IFN-γ Rα Chain (CD119)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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Anti-Human IFN-γ Rα Chain (CD119)