Mouse IgG2b Isotype Control (Clone MPC-11-mIgG2b) – Recombinant In vivo Purified No Carrier Protein

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This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

This recombinant isotype control antibody features the variable regions of clone MPC-11 coupled to an engineered Mouse IgG2b Fc domain and expressed in . Recombinant antibodies are manufactured in our animal free facility located in St. Louis, Missouri USA using only in vitro protein free HEK293 cells entirely without FBS which avoids serum-induced lot variability and bovine protein contamination, making it ideal for highly sensitive or in vivo assays. This antibody is then purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s recombinant biosimilar antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

Clone MPC-11 is a recombinant mouse IgG2b, kappa isotype control. Originally derived from a BALB/c mouse plasmacytoma, the variable regions of this clone possess no known functional specificity or reactivity toward mammalian antigens. This makes it an ideal negative control for establishing accurate baselines in flow cytometry, immunohistochemistry, and functional assays by accounting for non-specific background signal.

1.) Source/Vendor: Sigma-Aldrich / ECACC Catalog Number: 91031103 (or CB_91031103)