Recombinant Rat IL-1β

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Pricing & Details

Product. No.I-212
Alternate Names
Interleukin-1 Beta, IL1B, IL-1, IL1-Beta, IL1F2
Expression Host
E. coli
Species
Rat
Prod No.
Size
Price
Avail.
Qty
Add to cart
I-212-10 µg
10 µg
$299.00
In stock
Max:
Min: 1
Step: 1
I-212-50 µg
50 µg
$869.00
In stock
Max:
Min: 1
Step: 1

Background

IL-1R1 is an 80 kD transmembrane glycoprotein and a member of the Ig superfamily. IL-1 binds both IL-1R1 and IL-1R2. The ligands IL-1α and IL-1β only commence when binding IL-1R1 because IL-1R2 acts as a decoy receptor and competitively antagonizes the binding of IL-1α and IL-1β, thereby reducing their biological effects. Binding of the ligands to IL-1R1 is enhanced by an accessory protein, IL-1R-AcP. IL-1α can exist as either a 31 kD precursor or an 18 kD mature form. IL-1α is a proinflammatory cell-associated cytokine and IL-1β is a 17.5 kD proinflammatory secreted cytokine. Both IL-1α and IL-1β bind to the same receptor and have similar properties that include stimulation of thymocyte proliferation via IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. Furthermore, IL-1β mediates a variety of immune and inflammatory responses. IL-1β is a major cause of severe inflammation in the mouse model of Crohn’s Disease, which makes IL-1β an important target for this disease.

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.01 EU/µg as determined by the LAL method
Expression Host
E. coli
Protein Accession No.
Amino Acid Sequence
mvpir qlhcrlrdeq qkclvlsdpc elkalhlngq nisqqvvfsm sfvqgetsnd kipvalglkg lnlylscvmk dgtptlqles vdpkqypkkk mekrfvfnki evktkvefes aqfpnwyist sqaehrpvfl gnsngrdivd ftmepvss
N-terminal Sequence Analysis
Met
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Rat IL-1β is Mr 17 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is 18 kDa.
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in MES, EDTA, Dithiothreitol (DTT), sodium chloride (NaCl) and polyethylene glycol 8000 (PEG 8000).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Dinarello, CA. et al. (1996) Blood 87: 2095
2. Yuan, J. et al. (1993) Cell 75: 641
3. Maeda, S. et al. (2005) Science 4: 735
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.