Anti-Human IL-1β (Clone 8516)

Pricing & Details

Product No.I-629
Clone
8516
Protein
IL-1β
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Interleukin-1 Beta, IL1B, IL-1, IL1-Beta, IL1F2
Isotype
Mouse
IgG1
Applications
CyTOF®
,
FC
,
ICC
,
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
I-629-25 µg
25 µg
$230.00
In stock
Max:
Min: 1
Step: 1
I-629-100 µg
100 µg
$431.00
In stock
Max:
Min: 1
Step: 1
I-629-500 µg
500 µg
$917.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human IL-1β (Accession # P01584)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL-1. A suggested method would be to stain cells expressing IL-1 with 0.25 µl per 1-5 x 106 cells in a total staining volume of ≤ 200 µl followed by PN:M1189.
Western Blotting: To detect Human IL-1β this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-1β is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human IL-1β bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IL-1β (at 50 pg/ml) is 0.001 - 0.003 µg/ml.
Immunocytochemistry: Suitable for use at concentration of 8-25 µg/mL.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 8516 recognizes an epitope on human IL-1β. This antibody shows less than 5% cross-reactivity with rmIL-1β and rpIL-β and no cross-reactivity with rrIL-1β, rmIL-1α, rhIL-1ra, rmIL-1ra, or rrIL-1α.
Background
IL-1R1 is an 80 kD transmembrane glycoprotein and a member of the Ig superfamily. IL-1 binds both IL-1R1 and IL-1R2. The ligands IL-1α and IL-1β only commence when binding IL-1R1 because IL-1R2 acts as a decoy receptor and competitively antagonizes the binding of IL-1α and IL-1β, thereby reducing their biological effects. Binding of the ligands to IL-1R1 is enhanced by an accessory protein, IL-1R-AcP. IL-1α can exist as either a 31 kD precursor or an 18 kD mature form. IL-1α is a proinflammatory cell-associated cytokine and IL-1β is a 17.5 kD proinflammatory secreted cytokine. Both IL-1α and IL-1β bind to the same receptor and have similar properties that include stimulation of thymocyte proliferation via IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. Furthermore, IL-1β mediates a variety of immune and inflammatory responses. IL-1β is a major cause of severe inflammation in the mouse model of Crohn’s Disease, which makes IL-1β an important target for this disease.

Antigen Details

Protein
PubMed

References & Citations

1. Dinarello, CA. et al. (1996) Blood 87: 2095
2. Yuan, J. et al. (1993) Cell 75: 641
3. Maeda, S. et al. (2005) Science 4: 735
Flow Cytometry
General Western Blot Protocol

Formats Available

Prod No.
Description
I-629
Products are for research use only. Not for use in diagnostic or therapeutic procedures.