For cell sorting applications
Leinco Technologies’ HEPES buffered saline works great and is particularly useful for high-pressure cell sorting as HEPES controls the pH better at high pressure compared to phosphate buffers. We have also shown that for certain cell types, HEPES can help with post sort cell viability as well.
For Flow Cytometry Analysis Applications
Leinco’s sheath fluids for analysis contain mild preservatives to protect the instrumentation and core facility from contaminations. A detailed cleaning procedure with recommendations can be found in our Literature Library which should be considered along with the use of ClearFlow™ sheath fluid products to minimize the potential for any contaminations. For high throughput screening (HTC) instruments developed by companies like Propel, Bio-Rad, and BD, we have developed a great sheath fluid product with a surfactant, helping keep the system flowing by reducing the surface tension. All our sheath products are manufactured in our ISO 9001:2015 (class 10,000) biological clean rooms and are ultra-filtered to assure low particulate count (SEE BELOW EXPERIMENTAL DATA) for minimizing fluorescence background and guaranteeing a higher signal to noise ratio.