Data
Detection of 6-His tagged protein in 200, 100, and 50ng of E. coli lysate containing tagged fusion protein with #18814 diluted 1:25,000. - -
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Antibody DetailsProduct DetailsHost Species Rabbit Immunogen 6-His (HHHHHH) conjugated to KLH. Product Concentration 1.0 mg/ml Formulation Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide State of Matter Liquid Product Preparation Purified by peptide immuno-affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at 2-8°C. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at a dilution of 1:1,000-1:20,000.
ELISA: for detection, use at a dilution of 1:1,000-1:30,000; for coating, use at a dilution of 1:100- 1:500. Immunocytochemistry: use at a dilution of 1:100-1:400. Immunoprecipitation: use at 1-4ug/mg lysate These are recommended dilutions. Endusers should determine optimal dilutions for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Antibody was affinity-purified on the peptide. This antibody does not recognize all N-terminal 6-His tagged proteins. Background A polyhistidine-tag consists of at least six histidine (His) residues, often at the N- or C-terminus of a protein. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems. Antigen DetailsResearch Area Epitope Tag References & CitationsTechnical Protocols ICC   |
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